Palm Fruit Bioactives modulate human astrocyte activity in vitro altering the cytokine secretome reducing levels of TNFα, RANTES and IP-10
| Autor: | T. G. Sambandan, Ravigadevi Sambanthamurthi, Anthony J. Sinskey, Vera V. Koledova, Chokyun Rha, Syed Fairus, Kirsten Schneider, Anastasia Artamonova, Gal Zeidman, Robert P Weinberg, Adlai Grayson |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemokine medicine.medical_treatment Interleukin-1beta lcsh:Medicine Arecaceae Article CCL5 03 medical and health sciences 0302 clinical medicine medicine Humans lcsh:Science Cell adhesion Chemokine CCL5 Multidisciplinary biology Plant Extracts Tumor Necrosis Factor-alpha Chemistry lcsh:R Interleukin Intercellular adhesion molecule Cell biology Chemokine CXCL10 030104 developmental biology Cytokine medicine.anatomical_structure Astrocytes Fruit biology.protein lcsh:Q Neural cell adhesion molecule Reactive Oxygen Species 030217 neurology & neurosurgery Astrocyte |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-19 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-34763-3 |
| Popis: | Neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease, are becoming more prevalent and an increasing burden on society. Neurodegenerative diseases often arise in the milieu of neuro-inflammation of the brain. Reactive astrocytes are key regulators in the development of neuro-inflammation. This study describes the effects of Palm Fruit Bioactives (PFB) on the behavior of human astrocytes which have been activated by IL-1β. When activated, the astrocytes proliferate, release numerous cytokines/chemokines including TNFα, RANTES (CCL5), IP-10 (CXCL10), generate reactive oxygen species (ROS), and express specific cell surface biomarkers such as the Intercellular Adhesion Molecule (ICAM), Vascular Cellular Adhesion Molecule (VCAM) and the Neuronal Cellular Adhesion Molecule (NCAM). Interleukin 1-beta (IL-1β) causes activation of human astrocytes with marked upregulation of pro-inflammatory genes. We show significant inhibition of these pro-inflammatory processes when IL-1β-activated astrocytes are exposed to PFB. PFB causes a dose-dependent and time-dependent reduction in specific cytokines: TNFα, RANTES, and IP-10. We also show that PFB significantly reduces ROS production by IL-1β-activated astrocytes. Furthermore, PFB also reduces the expression of ICAM and VCAM, both in activated and naïve human astrocytes in vitro. Since reactive astrocytes play an essential role in the neuroinflammatory state preceding neurodegenerative diseases, this study suggests that PFB may have a potential role in their prevention and/or treatment. |
| Databáze: | OpenAIRE |
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