Effects of ovarian hormones and estrogen receptor α on physical activity and skeletal muscle fatigue in female mice

Autor: Gengyun Le, Christine A. Cabelka, Nardina Nash, Espen E. Spangenburg, Cory W. Baumann, Angus Lindsay, Brittany C. Collins, Dawn A. Lowe
Rok vydání: 2019
Předmět:
0301 basic medicine
Aging
Estrogen receptor
Biochemistry
Mice
Random Allocation
0302 clinical medicine
Endocrinology
Tandem Mass Spectrometry
Progesterone
computer.programming_language
Mice
Knockout
Estradiol
sed
Menopause
medicine.anatomical_structure
Muscle Fatigue
Female
hormones
hormone substitutes
and hormone antagonists
Muscle Contraction
medicine.medical_specialty
Ovariectomy
Article
Contractility
03 medical and health sciences
Physical Conditioning
Animal
Internal medicine
Genetics
medicine
Animals
Muscle Strength
Muscle
Skeletal
Molecular Biology
Soleus muscle
business.industry
Estrogen Receptor alpha
Wild type
Skeletal muscle
Estrogens
Cell Biology
medicine.disease
Mice
Inbred C57BL
030104 developmental biology
Torque
Progestins
business
computer
030217 neurology & neurosurgery
Chromatography
Liquid
Hormone
Zdroj: Experimental Gerontology. 115:155-164
ISSN: 0531-5565
DOI: 10.1016/j.exger.2018.11.003
Popis: Menopause is associated with declines in physical activity and skeletal muscle strength. Physical activity is also reduced in rodents after ovariectomy (OVX) and whole-body estrogen receptor α (ERα) knockout. However, it is unclear if the effects are estradiol (E(2)) specific. Thus, the overall purpose of this study was to investigate the effects of the ovarian hormones, E(2) and progesterone (P4), and skeletal muscle ERα (skmERα) on physical activity and skeletal muscle contractility in female mice. METHODS: Study 1: Forty female C57Bl/6J mice were given free access to running wheels for 2 weeks to assess baseline running and randomized into 4 treatment groups: OVX, OVX+E(2), OVX+P4, OVX+E(2)+P4. All mice underwent OVX, returned to wheels for 2 weeks, received hormone pellet implants and returned to running wheels for 6 weeks, after which soleus muscle contractility testing was completed. Study 2: Thirty-two skeletal muscle specific ERα knock-out (skmERαKO) mice and wildtype (WT) littermates were randomized into 4 groups: skmERαKO-Run, skmERαWT-Run, skmERαKO-Sed, and skmERαWT-Sed. Run mice were given free access to wheels for 20 wk and sedentary (Sed) mice maintained normal cage activities. At the end point, muscle contractility was tested. RESULTS: Study 1: OVX+E(2)+P4 group ran greater distances than both the OVX and OVX+P4 groups (p≤0.009). After fatiguing contractions, soleus muscles of the OVX+E(2)+P4 group maintained greater submaximal force than those of other groups (p=0.023). Immediately after the fatiguing contractions, OVX+E(2)+P4 muscles had greater maximal force production than the OVX+E(2) group (p=0.027). Study 2: There were no differences in running distance between skmERαWT and skmERαKO mice (p=0.240). Soleus muscles of skmERαKO mice were more fatigable (p
Databáze: OpenAIRE
Externí odkaz: