A selfish DNA element engages a meiosis-specific motor and telomeres for germ-line propagation
| Autor: | Michael N. Conrad, David B. Kaback, Soumitra Sau, Michael E. Dresser, Makkuni Jayaram, Chih Ying Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Green Fluorescent Proteins Telomere-Binding Proteins Mitosis Cell Cycle Proteins Saccharomyces cerevisiae Spindle Apparatus Biology Prophase Article Shelterin Complex Chromosome segregation Plasmid Meiosis Genes Reporter Chromosome Segregation Gene Expression Regulation Fungal DNA Fungal Kinetochores Research Articles Repetitive Sequences Nucleic Acid Genetics Telomere-binding protein Fungal genetics Membrane Proteins Nuclear Proteins Cell Biology Telomere Spindle apparatus Cytoskeletal Proteins Chromosomes Fungal Plasmids Transcription Factors |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | The yeast 2 micron plasmid engages a meiosis-specific motor that orchestrates telomere-led chromosome movements for its telomere-associated segregation during meiosis I. The chromosome-like mitotic stability of the yeast 2 micron plasmid is conferred by the plasmid proteins Rep1-Rep2 and the cis-acting locus STB, likely by promoting plasmid-chromosome association and segregation by hitchhiking. Our analysis reveals that stable plasmid segregation during meiosis requires the bouquet proteins Ndj1 and Csm4. Plasmid relocalization from the nuclear interior in mitotic cells to the periphery at or proximal to telomeres rises from early meiosis to pachytene. Analogous to chromosomes, the plasmid undergoes Csm4- and Ndj1-dependent rapid prophase movements with speeds comparable to those of telomeres. Lack of Ndj1 partially disrupts plasmid–telomere association without affecting plasmid colocalization with the telomere-binding protein Rap1. The plasmid appears to engage a meiosis-specific motor that orchestrates telomere-led chromosome movements for its telomere-associated segregation during meiosis I. This hitherto uncharacterized mode of germ-line transmission by a selfish genetic element signifies a mechanistic variation within the shared theme of chromosome-coupled plasmid segregation during mitosis and meiosis. |
| Databáze: | OpenAIRE |
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