Tyramine and tryptamine as ligands for medical and biotechnological affinity sorbents
| Autor: | M. I. Afanas’eva, E. D. Ovchinnikova, D. A. Frid, S. N. Pokrovskii, P. A. Levashov, A. A. Az’muko, I. Yu. Adamova |
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| Rok vydání: | 2015 |
| Předmět: |
Tryptamine
Sorbent Chromatography biology Organic Chemistry Tyramine Sorption Ligands Biochemistry Chromatography Affinity Tryptamines Immunoglobulin A Lipoproteins LDL chemistry.chemical_compound chemistry Immunoglobulin G Blood plasma biology.protein Humans Bioorganic chemistry Antibody Lipoprotein(a) Lipoprotein |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1608-330X 1068-1620 |
| DOI: | 10.1134/s1068162015010112 |
| Popis: | A new procedure for the preparation of affinity sorbents based on tyramine and tryptamine ligands has been proposed. Sorption characteristics for the developed chromatographic materials were investigated. It was shown that tryptamine-sepharose and tyramine-sepharose efficiently bind IgG, IgA, lipoprotein (a) (Lp(a)) and low density lipoproteins (LDL) from blood plasma. The sorption capacity was 4–9 mg of IgG, 2–4 mg of IgA, 3–5 mg of Lp(a), and 5–7 mg of LDL per mL of gel. It was found that Lp(a) and IgG can bind to the sorbent either as themselves or as a complex of Lp(a) with IgG, the existence of which may indicate the presence of autoantibodies to Lp(a) in some patients. New sorbents are easy in synthesis, stable during use and storage, and they can be used for medical and biotechnological purposes to bind Lp(a), LDL, IgG, and IgA. |
| Databáze: | OpenAIRE |
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