Design of Protein Logic Gate System Operating on Lipid Membranes
| Autor: | Matic Kisovec, Gregor Anderluh, Saša Aden, Marjetka Podobnik, Neža Omersa |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Bacterial Toxins Biomedical Engineering Calorimetry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Permeability 03 medical and health sciences Synthetic biology Hemolysin Proteins lipidne membrane ddc:570 010608 biotechnology udc:577 listeriolizin O pore-forming toxin Lipid bilayer Heat-Shock Proteins Unilamellar Liposomes 030304 developmental biology protein logic gates 0303 health sciences Pore-forming toxin biokemija Chemistry pH Vesicle General Medicine Hydrogen-Ion Concentration Ankyrin Repeat Membrane DARPin Logic gate Drug delivery Biophysics Mutagenesis Site-Directed listeriolysin O Synthetic Biology lipid membrane pore Protein Binding Research Article |
| Zdroj: | ACS Synthetic Biology ACS synthetic biology, vol. 9, no. 2, pp. 316-328, 2020. ACS synthetic biology 9(2), 316-328 (2020). doi:10.1021/acssynbio.9b00340 |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/acssynbio.9b00340 |
| Popis: | ACS synthetic biology 9(2), 316 - 328 (2020). doi:10.1021/acssynbio.9b00340 Lipid membranes are becoming increasingly popular in synthetic biology due to their biophysical properties and crucial role in communication between different compartments. Several alluring protein–membrane sensors have already been developed, whereas protein logic gates designs on membrane-embedded proteins are very limited. Here we demonstrate the construction of a two-level protein–membrane logic gate with an OR-AND logic. The system consists of an engineered pH-dependent pore-forming protein listeriolysin O and its DARPin-based inhibitor, conjugated to a lipid vesicle membrane. The gate responds to low pH and removal of the inhibitor from the membrane either by switching to a reducing environment, protease cleavage, or any other signal depending on the conjugation chemistry used for inhibitor attachment to the membrane. This unique protein logic gate vesicle system advances generic sensing and actuator platforms used in synthetic biology and could be utilized in drug delivery. Published by ACS, Washington, DC |
| Databáze: | OpenAIRE |
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