Combining Adhesive Nanostructured Surfaces and Costimulatory Signals to Increase T Cell Activation
| Autor: | Joachim P. Spatz, Judith Guasch, Jennifer Diemer, Horst Kessler, Marco Hoffmann, Stefanie Neubauer, Hossein Riahinezhad |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CD3 Complex Surface Properties T-Lymphocytes T cell Cell Integrin Bioengineering Lymphocyte Activation Immunotherapy Adoptive 03 medical and health sciences chemistry.chemical_compound CD28 Antigens Coated Materials Biocompatible Cell Adhesion medicine Humans General Materials Science Cells Cultured Titanium biology Ionomycin Mechanical Engineering General Chemistry Condensed Matter Physics In vitro Nanostructures Transport protein Cell biology 030104 developmental biology medicine.anatomical_structure chemistry biology.protein Phorbol Tetradecanoylphorbol Acetate Gold Antibody Antibodies Immobilized Oligopeptides |
| Zdroj: | Nano Letters |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/acs.nanolett.8b02588 |
| Popis: | Adoptive cell therapies are showing very promising results in the fight against cancer. However, these therapies are expensive and technically challenging in part due to the need of a large number of specific T cells, which must be activated and expanded in vitro. Here we describe a method to activate primary human T cells using a combination of nanostructured surfaces functionalized with the stimulating anti-CD3 antibody and the peptidic sequence arginine-glycine-aspartic acid, as well as costimulatory agents (anti-CD28 antibody and a cocktail of phorbol 12-myristate 13-acetate, ionomycin, and protein transport inhibitors). Thus, we propose a method that combines nanotechnology with cell biology procedures to efficiently produce T cells in the laboratory, challenging the current state-of-the-art expansion methodologies. |
| Databáze: | OpenAIRE |
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