Neuronal calcium sensor-1 is expressed by dorsal root ganglion cells, is axonally transported to central and peripheral terminals, and is concentrated at nodes
| Autor: | Andreas Jeromin, John V. Priestley, Lesley G. Robson, S Averill |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Calcitonin Gene-Related Peptide Blotting Western Neuronal Calcium-Sensor Proteins Presynaptic Terminals Synaptophysin Calcitonin gene-related peptide Axonal Transport behavioral disciplines and activities Synaptic vesicle Dorsal root ganglion Ganglia Spinal Ranvier's Nodes mental disorders medicine Animals Rats Wistar Skin Neurons biology Chemistry General Neuroscience Calcium-Binding Proteins Neuropeptides Colocalization Spinal cord Immunohistochemistry Sciatic Nerve Rats Posterior Horn Cells medicine.anatomical_structure Spinal Cord nervous system Neuronal calcium sensor-1 biology.protein Axoplasmic transport Blood Vessels Neuroscience |
| Zdroj: | Neuroscience. 123:419-427 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2003.09.031 |
| Popis: | Neuronal calcium sensor-1 (NCS-1) is a member of the EF-hand calcium-binding protein superfamily which has been implicated in the modulation of a number of neuronal functions. In this study we have examined the expression of NCS-1 in adult rat dorsal root ganglion (DRG) neurons. NCS-1 immunoreactivity was present in most DRG neurons, including many calcitonin gene-related peptide (CGRP) expressing ones. NCS-1 showed some colocalization with the synaptic vesicle protein synaptophysin and underwent both anterograde and retrograde axonal transport. NCS-1 immunoreactivity was also present in the dorsal horn of the spinal cord, and in peripheral cutaneous terminals innervating blood vessels, where it was coexpressed with CGRP. In addition, NCS-1 in peripheral nerves was concentrated at nodes and adjoining paranodes. These results suggest novel roles for NCS-1, particularly in relation to channel function at nodes and to the peripheral release of vasoactive peptides. |
| Databáze: | OpenAIRE |
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