Abiotic Transformation of Toxaphene by Superreduced Vitamin B12 and Dicyanocobinamide
| Autor: | Anke Neumann, Steffen Ruppe, Walter Vetter, Gabriele Prof Dr Diekert |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Toxaphene
Insecticides Chromatography Gas biology Metabolite General Chemistry biology.organism_classification Vitamin B 12 chemistry.chemical_compound Transformation (genetics) Corrinoid chemistry Biotransformation Environmental chemistry Environmental Chemistry Cobamides Cyanocobalamin Corrinoids Oxidation-Reduction Water Pollutants Chemical Bacteria |
| Zdroj: | Environmental Science & Technology. 38:3063-3067 |
| ISSN: | 1520-5851 0013-936X |
| DOI: | 10.1021/es034994f |
| Popis: | Toxaphene is a complex organochlorine pesticide mixture, residues of which are widespread in the environment. Previous studies with the isolated bacterium Sulfurospirillum (formerly Dehalospirillum) multivorans resulted in an effective anaerobic biotransformation of toxaphene. Since the bacterium contains a corrinoid derivative in the active center of the tetrachloroethene dehalogenase, we attempted to use superreduced corrinoids for abiotic transformation of toxaphene. The two corrinoids studied were dicyanocobinamide and cyanocobalamin (vitamin B12). Superreduced dicyanocobinamide mediated a rapid transformation of toxaphene. More than 90% of the initial pool was transformed within 6 h. The transformation was nonselective, and even the most persistent metabolite in environmental samples, the so-called dead-end metabolite 2-exo,3-endo,6-exo,8,9,10-hexachlorobornane (B6-923 or Hx-Sed) was transformed within hours. Superreduced cyanocobalamin was also able to transform toxaphene albeit at significantly lower velocity. The lack of transformation products detectable in gas chromatograms of hexanes-extracted fractions of the assays suggests rapid, sequential dehalogenation and/or destruction of the C10-hydrocarbon backbone of the compounds of technical toxaphene. |
| Databáze: | OpenAIRE |
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