The DROOPING LEAF (DR) gene encoding GDSL esterase is involved in silica deposition in rice (Oryza sativa L.)
| Autor: | Mi-Ok Woo, Hee-Jong Koh, Yoye Yu, Piao Rihua |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Leaves Single Nucleotide Polymorphisms Mutant Plant Science 01 natural sciences Esterase Biochemistry chemistry.chemical_compound Database and Informatics Methods RNA interference Gene Expression Regulation Plant Cloning Molecular Plant Proteins Genetics Multidisciplinary Plant Anatomy Esterases Eukaryota food and beverages Plants Silicon Dioxide Nucleic acids Phenotypes Genetic interference Experimental Organism Systems Medicine Epigenetics Cellular Structures and Organelles Plant Cell Walls Cellular Types Sequence Analysis Research Article Inflorescences Ethyl methanesulfonate Bioinformatics Plant Cell Biology Science Mutagenesis (molecular biology technique) Biology Research and Analysis Methods 03 medical and health sciences Cell Walls Plant and Algal Models Plant Cells Grasses Panicles Allele Gene Oryza sativa Biology and life sciences Whole Genome Sequencing fungi Wild type Organisms Oryza Cell Biology Plant Leaves 030104 developmental biology chemistry Amino Acid Substitution Mutation Animal Studies RNA Gene expression Rice Sequence Alignment 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 15, Iss 9, p e0238887 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Leaf morphology is one of the most important agronomic traits in rice breeding because of its contribution to crop yield. The drooping leaf (dr) mutant was developed from the Ilpum rice cultivar by ethyl methanesulfonate (EMS) mutagenesis. Compared with the wild type, dr plants exhibited drooping leaves accompanied by a small midrib, short panicle, and reduced plant height. The phenotype of the dr plant was caused by a mutation within a single recessive gene on chromosome 2, dr (LOC_Os02g15230), which encodes a GDSL esterase. Analysis of wild-type and dr sequences revealed that the dr allele carried a single nucleotide substitution, glycine to aspartic acid. RNAi targeted to LOC_Os02g15230 produced same phenotypes to the dr mutation, confirming LOC_Os02g15230 as the dr gene. Microscopic observations and plant nutrient analysis of SiO2 revealed that silica was less abundant in dr leaves than in wild-type leaves. This study suggests that the dr gene is involved in the regulation of silica deposition and that disruption of silica processes lead to drooping leaf phenotypes. |
| Databáze: | OpenAIRE |
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