Radioprotective Effects of Heat-Killed Mycobacterium Tuberculosis in Cultured Cells and Radiosensitive Tissues

Autor: Jicong Du, Yang Xu, Yanyong Yang, Fu Gao, Bailong Li, Guo Jiaming, Ying Cheng, Jianming Cai, Jianguo Cui, Xiao Lei, Chen Yuanyuan, Cong Liu, Jintao Ju
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Hot Temperature
Radioprotection
Physiology
CD34
Antigens
CD34
Apoptosis
Cell Count
Radiation Tolerance
lcsh:Physiology
0302 clinical medicine
Radiation
Ionizing
Testis
Tissues injury
lcsh:QD415-436
Cells
Cultured
Mice
Inbred BALB C
medicine.diagnostic_test
lcsh:QP1-981
NF-kappa B
Protein Transport
Haematopoiesis
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cytokines
Stem cell
HKMT
Cell Survival
MAP Kinase Signaling System
Radiation-Protective Agents
Biology
Flow cytometry
lcsh:Biochemistry
03 medical and health sciences
Th2 Cells
Radioresistance
medicine
Animals
Viability assay
Radiation Injuries
Inflammation
Mycobacterium tuberculosis
Th1 Cells
Hematopoietic Stem Cells
Toll like receptor 2 (TLR2)
Molecular biology
030104 developmental biology
Immunology
Bone marrow
Zdroj: Cellular Physiology and Biochemistry, Vol 40, Iss 3-4, Pp 716-726 (2016)
ISSN: 1421-9778
1015-8987
Popis: Background: Exposure to ionizing radiation (IR) often causes severe damage to radiosensitive tissues, which limits the use of radiotherapy in cancer patients. Novel safe and effective radioprotectant is urgently required. It has been reported toll like receptor 2 (TLR2) plays a critical role in radioresistance. In this study, we demonstrated the protective effects of Heat-Killed Mycobacterium tuberculosis (HKMT), a potent TLR2 agonist, against IR. Methods: Cell survival and apoptosis were determined by CCK-8 assay and Annexin V assay, respectively. An immunofluorescence staining assay was used to detect the translocation of nuclear faktor-kappa beta (NF-kB) p65. Tissue damage was evaluated by Haematoxilin-Eosin (HE) staining assay. We also used a flow cytometry assay to measure the number of nucleated cells and CD34+ hemopoietic stem cells in bone marrow. A western blot assay was used to detect the changes of proteins involving TLR signaling pathway. Results: We found that HKMT increased cell viability and inhibited cell apoptosis after irradiation. HKMT induced NF-kB translocation and activated Erk1/2, p38 signaling pathway. HKMT also protected bone marrow and testis from destruction. Radiation-induced decreases of nucleated cells and CD34+ hemopoietic stem cells in bone marrow were also inhibited by HKMT treatment. We found that radiation caused increase of inflammatory cytokines was also suppressed by HKMT. Conclusion: Our data showed that HKMT exhibited radioprotective effects in vivo and in vitro through activating NF-kB and MAPK signaling pathway, suggesting a potential of HKMT as novel radioprotector.
Databáze: OpenAIRE
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