Augmentation of sinecomitant immunity in mice by gamma-(9H-purine-6-yl)thiomethyl L-glutamate (6-MPG), a water-soluble derivative of 6-mercaptopurine
| Autor: | Naoko Narasaki, Shigeyuki Takeyama, Kenji Tsujihara, Tatsuo Kashida, Kazuaki Naito |
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| Rok vydání: | 1998 |
| Předmět: |
Purine
Male medicine.medical_specialty Cyclophosphamide Ratón Cell Transplantation medicine.medical_treatment Pharmaceutical Science Spleen Antineoplastic Agents chemistry.chemical_compound Mice Immunity Neutralization Tests Internal medicine medicine Animals Neoplasm Metastasis Pharmacology Chemotherapy Immunity Cellular Mice Inbred BALB C business.industry Mercaptopurine Mitomycin C General Medicine Neoplasms Experimental Molecular biology medicine.anatomical_structure Endocrinology Phenotype chemistry business Neoplasm Transplantation medicine.drug Methylcholanthrene |
| Zdroj: | Biologicalpharmaceutical bulletin. 21(1) |
| ISSN: | 0918-6158 |
| Popis: | Effects of the administration of γ-(9H-purine-6-yl)thiomethyl L-glutamate (6-MPG), a water-soluble derivative of 6-mercaptopurine, on concomitant and sinecomitant immunity against the implanted MethA tumor were studied in BALB/C mice. In the concomitant immunity experiments, mice were intradermally inoculated with 1 x 10 5 MethA cells at the right inguinal region on day 0. In sinecomitant immunity experiments, mice were similarly inoculated on day -21, and the grown tumor was excised on day -11. Both the tumor-bearing and tumor-ectomized animals were re-inoculated with 3 x 10 6 MethA cells intradermally. at the left inguinal region on day. 10. Administration of 6-MPG (100 mg/kg, i.p.) on days 3 through 7 significantly inhibited growth of the re-inoculated tumor in both series of experiments. Cyclophosphamide, adriamycin, mitomycin C and cis-diamminedichloroplatinum (II) had no significant effect on the growth of the re-inoculated tumor in the tumor-ectomized mice. Spleen cells harvested from the 6-MPG-treated tumor-ectomized mice showed a strong tumor-neutralizing activity (Winn assay). |
| Databáze: | OpenAIRE |
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