Morphological and phenotypical features of ovarian metastases in breast cancer patients

Autor: Peter J. K. Kuppen, Vincent T.H.B.M. Smit, Bertine W. Huisman, Merle A. van der Steen, Carina G. J. M. Hilders, Cornelis F. M. Sier, Alexander L. Vahrmeijer, Inge T. A. Peters, J. Baptist Trimbos
Jazyk: angličtina
Rok vydání: 2017
Předmět:
tumor-specific imaging
Cancer Research
Pathology
endocrine system diseases
Receptor
ErbB-2
Cohort Studies
chemistry.chemical_compound
0302 clinical medicine
Carcinoembryonic antigen
Surgical oncology
Medicine
Ovarian tissue cryopreservation
Fertility preservation
Ovarian Neoplasms
030219 obstetrics & reproductive medicine
biology
ovarian tissue autotransplantation
Epithelial cell adhesion molecule
Cadherins
Epithelial Cell Adhesion Molecule
Immunohistochemistry
female genital diseases and pregnancy complications
3. Good health
medicine.anatomical_structure
Oncology
tumor markers
030220 oncology & carcinogenesis
Female
Research Article
Adult
medicine.medical_specialty
fertility preservation
Ovary
Breast Neoplasms
Sensitivity and Specificity
03 medical and health sciences
Breast cancer
breast cancer
ovarian metastases
Genetics
Biomarkers
Tumor
Humans
business.industry
medicine.disease
Carcinoembryonic Antigen
chemistry
biology.protein
business
Zdroj: BMC Cancer
BMC Cancer, 17
DOI: 10.1186/s12885-017-3191-y
Popis: Background Autotransplantation of frozen-thawed ovarian tissue is a method to preserve ovarian function and fertility in patients undergoing gonadotoxic therapy. In oncology patients, the safety cannot yet be guaranteed, since current tumor detection methods can only exclude the presence of malignant cells in ovarian fragments that are not transplanted. We determined the need for a novel detection method by studying the distribution of tumor cells in ovaries from patients with breast cancer. Furthermore, we examined which cell-surface proteins are suitable as a target for non-invasive tumor-specific imaging of ovarian metastases from invasive breast cancer. Methods Using the nationwide database of the Dutch Pathology Registry (PALGA), we identified a cohort of 46 women with primary invasive breast cancer and ovarian metastases. The localization and morphology of ovarian metastases were determined on hematoxylin-and-eosin-stained sections. The following cell-surface markers were immunohistochemically analyzed: E-cadherin, epithelial membrane antigen (EMA), human epidermal growth receptor type 2 (Her2/neu), carcinoembryonic antigen (CEA), αvβ6 integrin and epithelial cell adhesion molecule (EpCAM). Results The majority of ovarian metastases (71%) consisted of a solitary metastasis or multiple distinct nodules separated by uninvolved ovarian tissue, suggesting that ovarian metastases might be overlooked by the current detection approach. Combining the targets E-cadherin, EMA and Her2/neu resulted in nearly 100% detection of ductal ovarian metastases, whereas the combination of EMA, Her2/neu and EpCAM was most suitable to detect lobular ovarian metastases. Conclusions Examination of the actual ovarian transplants is recommended. A combination of targets is most appropriate to detect ovarian metastases by tumor-specific imaging. Electronic supplementary material The online version of this article (doi:10.1186/s12885-017-3191-y) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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