Frequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: a multicentre study

Autor: Beyza Ener, Çağrı Ergin, Dolunay Gülmez, Harun Ağca, Melek Tikveşli, Seçil Ak Aksoy, Müşerref Otkun, Ali Korhan Siğ, Dilara Öğünç, Betil Özhak, Tuncay Topaç, Aslı Özdemir, Dilek Yeşim Metin, Süleyha Hilmioğlu Polat, Yasemin Öz, Nedret Koç, Mustafa Altay Atalay, Zayre Erturan, Asuman Birinci, Nilgün Çerikçioğlu, Demet Timur, Fahriye Ekşi, Gonca Erköse Genç, Duygu Findik, Şaban Gürcan, Ayşe Kalkanci, Sevtap Arikan-Akdagli
Přispěvatelé: ENER B., ERGİN Ç., GÜLMEZ KIVANÇ D., AĞCA H., Tikvesli M., AKSOY S., Otkun M., Sig A. K., Ogunc D., ÖZHAK B., et al.
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Azoles
Antifungal Agents
Turkey
Mikrobiyoloji
fungal protein
Pharmacy
Sağlık Bilimleri
Turkey (republic)
fungus mutation
FARMAKOLOJİ VE ECZACILIK
antifungal agent
genetics
Pharmacology (medical)
gene mutation
fungal gene
General Pharmacology
Toxicology and Pharmaceutics
PHARMACOLOGY & PHARMACY
Pharmacology
Toxicology and Pharmaceutics (miscellaneous)
PHARMACOLOGY & TOXICOLOGY
Temel Bilimler
Basic Pharmaceutics Sciences
microsatellite marker
Life Sciences
clinical trial
antifungal resistance
itraconazole
microbial sensitivity test
Infectious Diseases
BULAŞICI HASTALIKLAR
Farmakoloji ve Toksikoloji
Natural Sciences
prospective study
Microbiology (medical)
MICROBIOLOGY
prevalence
Immunology
Life Sciences (LIFE)
Microbial Sensitivity Tests
minimum inhibitory concentration
Article
Fungal Proteins
pyrrole
turkey (bird)
Drug Resistance
Fungal
Drug Guides
Yaşam Bilimleri
Health Sciences
voriconazole
Aspergillosis
Humans
controlled study
human
fungus isolation
Eczacılık
molecular phylogeny
Pharmacology
nonhuman
İmmünoloji
General Immunology and Microbiology
screening
Aspergillus fumigatus
fungal strain
Pharmacology and Therapeutics
posaconazole
fungus growth
multicenter study
genotyping
Temel Eczacılık Bilimleri
Yaşam Bilimleri (LIFE)
pyrrole derivative
Popis: Objectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.
Bursa Uludag University Scientific Research Projects Commission [QUAP[T]-2015-5]; Ener Private Health Service Company
This work was partly supported by Bursa Uludag University Scientific Research Projects Commission (QUAP[T]-2015-5) and Ener Private Health Service Company.
Databáze: OpenAIRE
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