Chemoenzymatic Design of Heparan Sulfate Oligosaccharides*
| Autor: | Michel Weïwer, Arlene S. Bridges, Paul L. DeAngelis, Jian Liu, Renpeng Liu, Miao Chen, Qisheng Zhang, Xianxuan Zhou, Robert J. Linhardt, Yongmei Xu |
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| Rok vydání: | 2010 |
| Předmět: |
Sulfotransferase
Glycan Molecular Sequence Data Disaccharide Oligosaccharides Glycobiology and Extracellular Matrices Disaccharides Protein Engineering Polysaccharide Biochemistry Antithrombins chemistry.chemical_compound Sulfation Polysaccharides medicine Humans Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification biology Cell Biology Heparan sulfate Heparin Oligosaccharide Protein Structure Tertiary carbohydrates (lipids) Chemistry Kinetics Carbohydrate Sequence chemistry biology.protein Heparitin Sulfate Carbohydrate Epimerases medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 285:34240-34249 |
| ISSN: | 0021-9258 |
| Popis: | Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a disaccharide building block using glycosyltransferases, heparan sulfate C(5)-epimerase, and sulfotransferases. This method offers a generic approach to prepare heparan sulfate oligosaccharides possessing predictable structures. |
| Databáze: | OpenAIRE |
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