Development of a chemiluminescence-based quantitatve lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene

Autor: Angel Montoya, Mara Mirasoli, Patrizia Simoni, Luisa Stella Dolci, Angela Buragina, Massimo Guardigli, Stefano Girotti, Aldo Roda, Elisabetta Maiolini
Přispěvatelé: Mirasoli M., Buragina A., Dolci L.S., Guardigli M., Simoni P., Montoya A., Maiolini E., Girotti S., Roda A.
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Product recovery
Chemiluminescence
Portable device
Real samples
Limit of detection
Environmental pollution
Imaging techniques
Chemoluminescence
High specificity
Biochemistry
2
4
6-trinitrotoluene
Immunoreagents
Antigen-antibody binding
Analytical Chemistry
law.invention
Imaging
Assays
law
Cost benefit analysis
Explosives detection
Naked-eye
Quantitative analysis
Spectroscopy
Accuracy
Priority journal
Immunoassay
medicine.diagnostic_test
Chemistry
Environmental pollutions
Flow measurement
2
4
6-Trinitrotoluene
Sensitivity and specificity
Colloidal gold
Calibration
Bioanalytical methods
6-TRINITROTOLUENE (TNT)
Chemiluminescence detection
Process development
Bioanalysis
Ease of use
Imaging device
Nanotechnology
Highly sensitive
Article
Antibodies
Signal measurement
TECNOLOGIA ELECTRONICA
Semi-quantitative
Lateral flow immunoassay
Latex beads
medicine
Thermoelectrically-cooled
Environmental Chemistry
Colloids
Signal noise ratio
Detection limit
Dynamic range
Chromatography
Field study
Kinetics
Biosensors
Low sensitivity
Concentration (parameters)
Luminescent Measurements
Naked eye
Prediction
Biosensor
Controlled study
On-site analysis
Trinitrotoluene
Process optimization
Signal detection
Zdroj: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
DOI: 10.1016/j.aca.2012.01.036
Popis: [EN] Simple, rapid and highly sensitive assays, possibly allowing on-site analysis, are required in the security and forensic fields or to obtain early signs of environmental pollution. Several bioanalytical methods and biosensors based on portable devices have been developed for this purpose. Among them, Lateral Flow ImmunoAssays (LFIAs) offer the advantages of rapidity and ease of use and, thanks to the high specificity of antigen-antibody binding, allow greatly simplifying and reducing sample pre-analytical treatments. However, LFIAs usually employ colloidal gold or latex beads as labels and they rely on the formation of colored bands visible by the naked eye. With this assay format, only qualitative or semi-quantitative information can be obtained and low sensitivity is achieved. Recently, the use of enzyme-catalyzed chemiluminescence detection in LFIA has been proposed to overcome these problems. In this work, we describe the development of a quantitative CL-LFIA assay for the detection of 2,4,6-trinitrotoluene (TNT) in real samples. Thanks to the use of a portable imaging device for CL signal measurement based on a thermoelectrically cooled CCD camera, the analysis could be performed directly on-field. A limit of detection of 0.2¿gmL -1 TNT was obtained, which is five times lower than that obtained with a previously described colloidal gold-based LFIA developed employing the same immunoreagents. The dynamic range of the assay extended up to 5¿gmL -1 TNT and recoveries ranging from 97% to 111% were obtained in the analysis of real samples (post blast residues obtained from controlled explosion). © 2012 Elsevier B.V.
This work was supported by grants from the Italian Ministry of Instruction, University and Research (PRIN 2007AWK85F "Development of integrated analytical instruments based on luminescence principles for multiplexed analysis in POCT (Point-Of-Care Testing) applications" and PRIN 2006033429 "New Analytical Tools for Security and Criminal Investigations: Trace Detection and Identification of Explosives and Related Compounds") and from the University of Bologna ("Fundamental Oriented Research").
Databáze: OpenAIRE
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