Persistent increase in ecto‑5'‑nucleotidase activity from encephala of adult zebrafish exposed to ethanol during early development

Autor: Julia Huppes Majolo, Luiza Reali Nazario, Stefani Altenhofen, Talita Carneiro Brandão Pereira, Aline Haab Lutte, Rosane Souza da Silva, Maurício Reis Bogo, Adilio da Silva Dadda
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Embryo
Nonmammalian
Nonmammalian/drug effects
Embryo
Nonmammalian/drug effects
Dopamine
Acid Phosphatase
Biology
Toxicology
5'-nucleotidase
Andrology
03 medical and health sciences
Cellular and Molecular Neuroscience
Glutamatergic
0302 clinical medicine
Adenosine deaminase
Developmental Neuroscience
Pregnancy
medicine
Animals
Behavior
Animal/drug effects
Zebrafish
5'-Nucleotidase
Behavior
Brain/drug effects
Behavior
Animal
Ethanol
Acid Phosphatase/drug effects
Dopaminergic
Purinergic receptor
fungi
Brain
Animal/drug effects
biology.organism_classification
Adenosine
Dopamine/metabolism
030104 developmental biology
Embryo
Prenatal Exposure Delayed Effects
Pharyngula
biology.protein
Female
Zebrafish/embryology
Ethanol/pharmacology
5'-Nucleotidase/metabolism
Prenatal Exposure Delayed Effects/metabolism
030217 neurology & neurosurgery
medicine.drug
Zdroj: Neurotoxicology and Teratology, 70, 60-66
ISSN: 0892-0362
Popis: Prenatal alcohol exposure causes alterations to the brain and can lead to numerous cognitive and behavioral outcomes. Long-lasting effects of early ethanol exposure have been registered in glutamatergic and dopaminergic systems. The purinergic system has been registered as an additional target of ethanol exposure. The objective of this research was to evaluate if the ecto‑5'‑nucleotidase and adenosine deaminase activities and gene expression of adult zebrafish exposed to 1% ethanol during early development could be part of the long-lasting targets of ethanol. Zebrafish embryos were exposed to 1% ethanol in two distinct developmental phases: gastrula/segmentation (5-24 h post-fertilization) or pharyngula (24-48 h post-fertilization). At the end of three months, after checking for morphological outcomes, the evaluation of enzymatic activity and gene expression was performed. Exposure to ethanol did not promote gross morphological defects; however, a significant decrease in the body length was observed (17% in the gastrula and 22% in the pharyngula stage, p 0.05). Although the mechanism underlying these findings requires further investigation, these results indicate that ethanol exposure during restricted periods of brain development can have long-term consequences on ecto‑5'‑nucleotidase activity, which could have an impact on subtle sequels of ethanol early exposure.
Databáze: OpenAIRE
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