A novel voxel-wise lesion segmentation technique on 3.0-T diffusion MRI of hyperacute focal cerebral ischemia at 1 h after permanent MCAO in rats
| Autor: | Chi Hoon Choi, Chulhyun Lee, Kyung Sik Yi, Sang-Hoon Cha, Sang-Rae Lee, Jinwoo Hwang, Hong Jun Lee, Sung Sik Choi, Youngjeon Lee, Chang-Yeop Jeon |
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| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_specialty Ischemia computer.software_genre Brain Ischemia 030218 nuclear medicine & medical imaging Rats Sprague-Dawley Brain ischemia 03 medical and health sciences 0302 clinical medicine Neuroimaging Voxel Image Processing Computer-Assisted medicine Animals Acute stroke Lesion segmentation business.industry Brain Infarction Middle Cerebral Artery Original Articles medicine.disease Rats Diffusion Magnetic Resonance Imaging Neurology Neurology (clinical) Radiology Cardiology and Cardiovascular Medicine business computer Algorithms 030217 neurology & neurosurgery Diffusion MRI |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism. 38:1371-1383 |
| ISSN: | 1559-7016 0271-678X |
| Popis: | To assess hyperacute focal cerebral ischemia in rats on 3.0-Tesla diffusion-weighted imaging (DWI), we developed a novel voxel-wise lesion segmentation technique that overcomes intra- and inter-subject variation in apparent diffusion coefficient (ADC) distribution. Our novel technique involves the following: (1) intensity normalization including determination of the optimal type of region of interest (ROI) and its intra- and inter-subject validation, (2) verification of focal cerebral ischemic lesions at 1 h with gross and high-magnification light microscopy of hematoxylin-eosin (H&E) pathology, (3) voxel-wise segmentation on ADC with various thresholds, and (4) calculation of dice indices (DIs) to compare focal cerebral ischemic lesions at 1 h defined by ADC and matching H&E pathology. The best coefficient of variation was the mode of the left hemisphere after normalization using whole left hemispheric ROI, which showed lower intra- (2.54 ± 0.72%) and inter-subject (2.67 ± 0.70%) values than the original. Focal ischemic lesion at 1 h after middle cerebral artery occlusion (MCAO) was confirmed on both gross and microscopic H&E pathology. The 83 relative threshold of normalized ADC showed the highest mean DI (DI = 0.820 ± 0.075). We could evaluate hyperacute ischemic lesions at 1 h more reliably on 3-Tesla DWI in rat brains. |
| Databáze: | OpenAIRE |
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