Impact of a large deletion in the neuraminidase protein identified in a laninamivir‐selected influenza A/Brisbane/10/2007 (H3N2) variant on viral fitness in vitro and in ferrets

Autor:	Xavier Bouhy, Karen Dubé, Edith Beaulieu, Yacine Abed, Corey P. Mallett, Marie-Ève Hamelin, Julie Ann, Guy Boivin, Marie-Hélène Joly, Julie Carbonneau
Rok vydání:	2016
Předmět:	0301 basic medicine Pulmonary and Respiratory Medicine Epidemiology viruses 030106 microbiology Neuraminidase Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Virus Replication medicine.disease_cause Antiviral Agents Guanidines Virus resistance 03 medical and health sciences Short Article 0302 clinical medicine Zanamivir Orthomyxoviridae Infections Drug Resistance Viral Influenza Human medicine Animals Humans deletion 030212 general & internal medicine Pyrans Infectivity Mutation biology Influenza A Virus H3N2 Subtype Ferrets Public Health Environmental and Occupational Health Laninamivir Virology Infectious Diseases Viral replication Sialic Acids biology.protein RNA Viral Genetic Fitness influenza A(H3N2) Gene Deletion medicine.drug
Zdroj:	Influenza and Other Respiratory Viruses
ISSN:	1750-2659 1750-2640
Popis:	Viral fitness of a laninamivir-selected influenza A/Brisbane/10/2007-like (H3N2) isolate (LRVp9) containing a 237-amino acid neuraminidase deletion and a P194L hemagglutinin mutation was evaluated in vitro and in ferrets. LRVp9 and the wild-type (WT) virus showed comparable replication kinetics in MDCK-ST6GalI cells. Cultured virus was recovered between days 2 and 5 post-infection in nasal washes (NW) from the 4 WT-infected ferrets whereas no virus was recovered from the LRVp9-infected animals. There was a ≥1 log reduction in viral RNA copies/μl of NW for LRVp9 compared to WT at most time points. The large neuraminidase deletion compromises viral infectivity in vivo.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c96af122a376b8746b6554f653a71a1e https://doi.org/10.1111/irv.12356 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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