Characterization and cytoprotective properties of Sargassum natans fucoidan against urban aerosol-induced keratinocyte damage
| Autor: | C. M. Nanayakkara, D.T.U. Abeytunga, Ilekuttige Priyan Shanura Fernando, Kalu Kapuge Asanka Sanjeewa, A.P.J.P. Vaas, WonWoo Lee, Hyo Geun Lee, Hyun-Soo Kim, You-Jin Jeon, Dae Sung Lee, Hondamuni I. De Silva |
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| Rok vydání: | 2020 |
| Předmět: |
Keratinocytes
Magnetic Resonance Spectroscopy Antioxidant DNA damage medicine.medical_treatment 02 engineering and technology Protective Agents medicine.disease_cause Biochemistry Fucose Cell Line 03 medical and health sciences chemistry.chemical_compound Polysaccharides Structural Biology Spectroscopy Fourier Transform Infrared medicine Humans Molecular Biology 030304 developmental biology Aerosols Air Pollutants 0303 health sciences Chemistry Fucoidan Cell Cycle Sargassum General Medicine Chromatography Ion Exchange 021001 nanoscience & nanotechnology Molecular Weight HaCaT medicine.anatomical_structure Cytoprotection Reactive Oxygen Species 0210 nano-technology Keratinocyte Intracellular Oxidative stress DNA Damage |
| Zdroj: | International Journal of Biological Macromolecules. 159:773-781 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.05.132 |
| Popis: | The escalation of fine particulate matter (PM) air pollution has recently become a global concern. Evidence is fast accumulating on PM exposure-related skin damage. The present study explored the therapeutic potentials of fucoidan purified from Sargassum natans against damaging effects of PM exposure on human HaCaT keratinocytes. Fucoidan (SNF7) was purified from S. natans by an enzyme-assisted extraction and purified by anion exchange chromatography. SNF7 (≈50 kDa) was identified as a fucoidan containing 70.97% fucose and 36.41 ± 0.59% of sulfate. Treatment of fine dust from Beijing, China (CFD) increased intracellular ROS levels in HaCaT cells triggering DNA damage and apoptosis. Treatment of SNF7 dose-dependently attenuated CFD-induced surge of intracellular ROS levels in keratinocytes by increasing antioxidant defense enzymes. Moreover, SNF7 chelated metal ions Pb2+, Ba2+, Sr2+, Cu2+, Fe2+, and Ca2+ coming from CFD. The results substantiated the potential therapeutic effects of SNF7 against CFD-induced oxidative stress. Further studies could promote SNF7's use as an active ingredient in cosmetics. |
| Databáze: | OpenAIRE |
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