Potent immunity to low doses of influenza vaccine by probabilistic guided micro-targeted skin delivery in a mouse model
| Autor: | Xianfeng Chen, Lorena E. Brown, Michael S. Roberts, Tarl W. Prow, Michael L. Crichton, Emily J. Fairmaid, Germain J. P. Fernando, Ian H. Frazer, Mark A. F. Kendall |
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| Přispěvatelé: | Fernando, Germain JP, Chen, Xianfeng, Prow, Tarl W, Crichton, Michael L, Fairmaid, Emily J, Roberts, Michael Stephen, Frazer, Ian H, Brown, Lorena E, Kendall, Mark AF |
| Rok vydání: | 2009 |
| Předmět: |
Microinjections
Influenza vaccine medicine.medical_treatment Science Injections Subcutaneous Antigen-Presenting Cells 02 engineering and technology Gene delivery Public Health and Epidemiology/Immunization 03 medical and health sciences Mice Antigen Immunity Medicine Animals Virology/Vaccines 030304 developmental biology Probability Skin 0303 health sciences Multidisciplinary business.industry Viral Vaccine 021001 nanoscience & nanotechnology Virology 3. Good health Immunity Humoral Vaccination Treatment Outcome Immunization Influenza Vaccines Immunology Models Animal Immunology/Immune Response nanopatch influenza vaccine 0210 nano-technology business Adjuvant Research Article Biotechnology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 4, p e10266 (2010) |
| ISSN: | 1932-6203 |
| Popis: | BackgroundOver 14 million people die each year from infectious diseases despite extensive vaccine use [1]. The needle and syringe--first invented in 1853--is still the primary delivery device, injecting liquid vaccine into muscle. Vaccines could be far more effective if they were precisely delivered into the narrow layer just beneath the skin surface that contains a much higher density of potent antigen-presenting cells (APCs) essential to generate a protective immune response. We hypothesized that successful vaccination could be achieved this way with far lower antigen doses than required by the needle and syringe.Methodology/principal findingsTo meet this objective, using a probability-based theoretical analysis for targeting skin APCs, we designed the Nanopatch, which contains an array of densely packed projections (21025/cm(2)) invisible to the human eye (110 microm in length, tapering to tips with a sharpness of Conclusions/significanceOur results represent a marked improvement--an order of magnitude greater than reported by others--for injected doses administered by other delivery methods, without reliance on an added adjuvant, and with only a single vaccination. This study provides a proven mathematical/engineering delivery device template for extension into human studies--and we speculate that successful translation of these findings into humans could uniquely assist with problems of vaccine shortages and distribution--together with alleviating fear of the needle and the need for trained practitioners to administer vaccine, e.g., during an influenza pandemic. |
| Databáze: | OpenAIRE |
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