Effect of Annexin A1 gene on the proliferation and invasion of esophageal squamous cell carcinoma cells and its regulatory mechanisms
| Autor: | Junxing Huang, Shengbin Dai, Lixin Zhang, Kaijin Lu, Gaohua Han, Yunyao Ye, Jun Ye |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Esophageal Neoplasms Cell Gene Expression Biology Transfection microRNA-196a 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor Genetics medicine Humans Annexin A1 Regulation of gene expression Oncogene Cell growth Articles General Medicine tumor invasion Cell cycle Cadherins esophageal squamous cell carcinoma Gene Expression Regulation Neoplastic MicroRNAs cell proliferation 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Carcinoma Squamous Cell Cancer research Snail Family Transcription Factors A431 cells |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2016.2840 |
| Popis: | The aim of this study was to examine the effect of Annexin A1 (ANXA1) on the proliferation, migration and invasion of esophageal squamous cell carcinoma (ESCC) cells and its possible mechanisms of action. After constructing the ANXA1 overexpression plasmid, we transfected this plasmid and/or microRNA (miRNA)-196a mimic into ESCC cells (Eca109 cell line). Methyl thiazolyl tetrazolium (MTT) assay and Transwell chamber assay were performed to determine cell proliferation, migration and invasion, respectively. Western blot analysis was used to examine the protein expression levels of ANXA1, Snail and E-cadherin. RT-PCR was used to detect the expression of miRNA-196a. Our results revealed that ANXA1 expression was upregulated in the cells transfected with the ANXA1 overexpression plasmid, and cell proliferation, migration and invasion were significantly increased (p=0.004, p |
| Databáze: | OpenAIRE |
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