Combined effects of high fat diet and exercise on autophagy in white adipose tissue of mice

Autor: Saeed, Daneshyar, Gholamreza, Tavoosidana, Mahdi, Bahmani, Saeed Shokati, Basir, Maryam, Delfan, Ismail, Laher, Ayoub, Saeidi, Urs, Granacher, Hassane, Zouhal
Přispěvatelé: Islamic Azad University of Hamedan, Tehran University of Medical Sciences (TUMS), University of Guilan, Alzahra University, University of British Columbia (UBC), University of Kurdistan [Sanandaj - Iran] (UOK), University of Freiburg [Freiburg], Laboratoire Mouvement Sport Santé (M2S), Université de Rennes (UR)-École normale supérieure - Rennes (ENS Rennes)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut International des Sciences du Sport (2I2S), This work was supported by Tehran University of Medical Sciences and Health Services (no. 47823) and University of Ayatollah Alozma Boroujerdi (no. 15664-214256).
Rok vydání: 2023
Předmět:
Zdroj: Life Sciences
Life Sciences, 2023, 314, pp.121335. ⟨10.1016/j.lfs.2022.121335⟩
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2022.121335
Popis: International audience; Aim: The effects of nutrition and exercise on autophagy are not well studied. This study aimed to investigate the combined effects of high-fat diets (HFD) and exercise training (ET) on autophagy in white adipose tissue of mice.Materials and methods: Male C57BL/6 mice were assigned into four groups of 7 mice per group: (1) Control, (2) high-fat diet-induced obesity (HFD-Ob), (3) exercise training (ET), and (4) high-fat diet with exercise training (HFD-ET). The HFD-Ob group was fed a high-fat diet for 14 weeks, while the ET group continuously ran on a treadmill for five sessions per week for seven weeks, and the HFD-ET group had both HFD and exercise training. qReal-time-PCR and western blot were used to measure the mRNA and protein levels of autophagy markers in white adipose tissue.Results: Mice from the HFD group showed higher levels in autophagy-related gene5 (ATG5, p = 0.04), ATG7 (p = 0.002), cathepsin B (CTSB, p = 0.0004), LC3-II (p = 0.03) than control. Mice in the ET group displayed higher levels of genes for ATG7 (p = 0.0003), microtubule-associated protein1-light chain 3 (LC3, p = 0.05), lysosome-associated membrane protein 2 (LAMP2, p = 0.04) and cathepsin L (CTSL, p = 0.03) than control. Mice from the HFD-ET group had higher levels of genes for ATG7 (p = 0.05) and CTSL (p = 0.043) and lower levels of genes for CTSB (p = 0.045) compared to the HFD group and lower levels of LAMP2 (p = 0.02) compared to the ET group.Conclusion: There were increases in autophagosome formation in the white adipose tissue from mice in the HFD and ET groups. A combination of HFD and ET enhances autophagosome formation and modulates lysosomal degradation in white adipose tissue.
Databáze: OpenAIRE
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