A new perspective on using glycols in glutamate biosensor design: from stabilizing agents to a new containment net
| Autor: | Pier Andrea Serra, Andrea Bacciu, Salvatore Marceddu, Gaia Giovanna Maria Rocchitta, Patrizia Monti, Giovanna Delogu, Paola Arrigo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
glutamate biosensor
glycols over-time stability containment net Excitotoxicity medicine.disease_cause 01 natural sciences Neopentyl glycol Analytical Chemistry lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glycerol medicine lcsh:QD415-436 Physical and Theoretical Chemistry Triethylene glycol 010401 analytical chemistry Glutamate receptor Diethylene glycol technology industry and agriculture 0104 chemical sciences chemistry Biophysics Biosensor 030217 neurology & neurosurgery Stabilizer (chemistry) |
| Zdroj: | Chemosensors 8 (2020): 1–16. doi:10.3390/chemosensors8020023 info:cnr-pdr/source/autori:Bacciu A, Arrigo P, Delogu G, Marceddu S, Monti P, Rocchitta G, Serra P A/titolo:A new perspective on using glycols in glutamate biosensor design: from stabilizing agents to a new containment net/doi:10.3390%2Fchemosensors8020023/rivista:Chemosensors/anno:2020/pagina_da:1/pagina_a:16/intervallo_pagine:1–16/volume:8 Chemosensors Volume 8 Issue 2 Chemosensors, Vol 8, Iss 2, p 23 (2020) |
| DOI: | 10.3390/chemosensors8020023 |
| Popis: | Glutamate is a major excitatory neurotransmitter in the brain. It is involved in many normal physiological brain activities, but also neurological disorders and excitotoxicity. Hence, glutamate measurement is important both in clinical and pre-clinical studies. Pre-clinical studies often use amperometric biosensors due to their low invasiveness and the relatively small size of the devices. These devices also provide fast, real-time measurements because of their high sensitivity. In the present study, diethylene glycol (DEG), neopentyl glycol (NPG), triethylene glycol (TEG), and glycerol (GLY) were used to increase the long-term stability of glutamate biosensors. The evaluation was made by measuring variations of the main enzymatic (VMAX and KM) and analytical (Linear Region Slope (LRS)) parameters. Of the glycols tested, TEG was the most promising stabilizer, showing about twice as high VMAX maintained over a greater duration than with other stabilizers tested. It is also yielded the most stable linear region slope (LRS) values over the study duration. Moreover, we highlighted the ability of glycols to interact with enzyme molecules to form a containment network, able to maintain all the layered components of the biosensor adhering to the transducer. |
| Databáze: | OpenAIRE |
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