Detection of the synthetic drug 4-fluoroamphetamine (4-FA) in serum and urine
| Autor: | T. Kaufmann, J. Röhrich, S. Zörntlein, R. Urban, Jürgen Prof. Dr. Becker |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Automobile Driving
Poison control Phenethylamines Urine Pharmacology Gas Chromatography-Mass Spectrometry Pathology and Forensic Medicine Designer Drugs 4-Fluoroamphetamine Forensic Toxicology Limit of Detection Medicine Humans Amphetamine Driving under the influence Detection limit Fluorocarbons medicine.diagnostic_test business.industry celebrities Amphetamines celebrities.reason_for_arrest Substance Abuse Detection Immunoassay business Law medicine.drug |
| Zdroj: | Forensic science international. 215(1-3) |
| ISSN: | 1872-6283 |
| Popis: | 4-Fluoroamphetamine (4-FA) was detected in the blood and urine of two individuals suspected for driving under the influence (DUI). The test for amphetamines in urine subjected to immunoassay screening using the CEDIA DAU assay proved positive. Further investigations revealed a 4-FA cross-reactivity of about 6% in the CEDIA amphetamine assay. 4-FA was qualitatively detected in a general unknown screening for drugs using GC/MS in full scan mode. No other drugs or fluorinated phenethylamines were detected. A validated GC/MS method was established in SIM mode for serum analysis of 4-FA with a limit of detection (LOD) of 1 ng/mL and a lower limit of quantification (LLOQ) of 5 ng/mL. Intra-assay precision was approx. 4% and inter-assay precision approx. 8%. Applying this method, the 4-FA serum concentrations of the two subjects were determined to be 350 ng/mL and 475 ng/mL, respectively. Given the pharmacological data of amphetamine, 4-FA psychoactive effects are to be expected at these serum levels. Both subjects exhibited sympathomimetic effects and psychostimulant-like impairment accordingly. |
| Databáze: | OpenAIRE |
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