Immunohistochemical localization of Toll-like receptors 1-10 in periodontitis

Autor: Mika Hukkanen, Riina Richardson, Yrjö T. Konttinen, Arzu Beklen
Rok vydání: 2008
Předmět:
Periodontium
Pathology
Alveolar Bone Loss
Gingiva
Epithelium
Pathogenesis
0302 clinical medicine
0303 health sciences
Toll-like receptor
Dental Plaque Index
Toll-Like Receptors
Middle Aged
Immunohistochemistry
medicine.anatomical_structure
Connective Tissue
Periodontal Index
medicine.symptom
Adult
Microbiology (medical)
medicine.medical_specialty
Immunology
Connective tissue
Inflammation
Biology
Microbiology
03 medical and health sciences
Periodontal Attachment Loss
medicine
Humans
Periodontal Pocket
Periodontitis
General Dentistry
030304 developmental biology
030206 dentistry
medicine.disease
Toll-Like Receptor 1
Chronic periodontitis
Toll-Like Receptor 2
Toll-Like Receptor 3
Toll-Like Receptor 4
Toll-Like Receptor 5
Toll-Like Receptor 6
Toll-Like Receptor 7
Toll-Like Receptor 8
Toll-Like Receptor 10
Toll-Like Receptor 9
Chronic Disease
Gingival Hemorrhage
Zdroj: Oral Microbiology and Immunology. 23:425-431
ISSN: 1399-302X
0902-0055
DOI: 10.1111/j.1399-302x.2008.00448.x
Popis: BACKGROUND/AIM: In periodontitis, bacteria and pathogen-associated molecular patterns are sensed by Toll-like receptors (TLRs), which initiate intracellular signaling cascades that may lead to host inflammation. In this study, the expression and distribution of TLRs (TLR-1 to TLR-10) were immunohistochemically detected in gingival epithelium and connective tissue. METHODS: Immunohistochemistry was used for the localization of TLRs in gingival tissue samples from 10 patients with chronic periodontitis and 10 healthy controls; these samples were obtained during routine periodontal flap operations and during extraction operations performed for retained wisdom teeth, respectively. For the evaluation, epithelial layers were stratified to basal, spinous, and superficial layers, and the percentages of TLR-positive cells were determined. RESULTS: Both healthy and periodontitis gingival tissues expressed all TLRs except TLR-10. In patients with periodontitis, epithelial cells showed increased TLR expression towards the basal layer. Healthy controls showed more variable cellular TLR expression and distribution between the layers of epithelium. In the connective tissue, consistently higher TLR expression was found within the periodontitis group compared to the healthy group. CONCLUSIONS: For the first time, the cellular expression and distribution of TLR-1 to TLR-10 have been studied in periodontitis, indicating that TLR-1 to TLR-9 are differentially expressed both in connective tissue and epithelial layers. Except for TLR-7 and TLR-8, all the other TLRs showed statistically significant differences between patients with periodontitis and healthy controls, suggesting their involvement in the pathogenesis of periodontitis.
Databáze: OpenAIRE
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