Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle
| Autor: | Tatsuro Egawa, Shingo Yokoyama, Yuki Matsui, Takafumi Ito, Hikari Kaneko, Katsumasa Goto, Koki Ando, Akiko Oyama, Yohei Suda, Yoshitaka Ohno |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Population lcsh:TX341-641 Cardiotoxins Article Muscle hypertrophy Cell Line Myoblasts Sodium Lactate 03 medical and health sciences chemistry.chemical_compound Mice Random Allocation 0302 clinical medicine Oral administration Internal medicine medicine Sodium lactate Animals skeletal muscle education Muscle Skeletal education.field_of_study lactate Nutrition and Dietetics Chemistry Myogenesis muscle satellite cell Regeneration (biology) Skeletal muscle 030229 sport sciences Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Endocrinology regeneration hypertrophy C2C12 lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Nutrients, Vol 11, Iss 4, p 869 (2019) Volume 11 Issue 4 |
| ISSN: | 2072-6643 |
| Popis: | The effects of lactate on muscle mass and regeneration were investigated using mouse skeletal muscle tissue and cultured C2C12 cells. Male C57BL/6J mice were randomly divided into (1) control, (2) lactate (1 mol/L in distilled water, 8.9 mL/g body weight)-administered, (3) cardio toxin (CTX)-injected (CX), and (4) lactate-administered after CTX-injection (LX) groups. CTX was injected into right tibialis anterior (TA) muscle before the oral administration of sodium lactate (five days/week for two weeks) to the mice. Oral lactate administration increased the muscle weight and fiber cross-sectional area, and the population of Pax7-positive nuclei in mouse TA skeletal muscle. Oral administration of lactate also facilitated the recovery process of CTX-associated injured mouse TA muscle mass accompanied with a transient increase in the population of Pax7-positive nuclei. Mouse myoblast-derived C2C12 cells were differentiated for five days to form myotubes with or without lactate administration. C2C12 myotube formation with an increase in protein content, fiber diameter, length, and myo-nuclei was stimulated by lactate. These observations suggest that lactate may be a potential molecule to stimulate muscle hypertrophy and regeneration of mouse skeletal muscle via the activation of muscle satellite cells. |
| Databáze: | OpenAIRE |
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