Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes
| Autor: | Sandrine Demanèche, Olivier Abbe, Saïd Azza, Issam Hasni, Philippe Colson, Eric Chabriere, Philippe Decloquement, Amina Cherif Louazani, Anthony Fontanini, Nicholas Armstrong, Bernard La Scola |
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| Přispěvatelé: | Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Amoeba, R&D Dept, ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) food.ingredient [SDV]Life Sciences [q-bio] Proteomics Microbiology Legionella pneumophila Amoeba (genus) 03 medical and health sciences food proteomics Adherent Culture Virology Lipidomics Willaertia magna C2c Maky Axenic Cell adhesion lcsh:QH301-705.5 030102 biochemistry & molecular biology biology fungi biology.organism_classification 6. Clean water culture 030104 developmental biology lcsh:Biology (General) Biochemistry amoebas lipidomics metabolism Intracellular |
| Zdroj: | Microorganisms Microorganisms, MDPI, 2020, 8 (11), ⟨10.3390/microorganisms8111791⟩ Volume 8 Issue 11 Microorganisms, 2020, 8 (11), ⟨10.3390/microorganisms8111791⟩ Microorganisms, Vol 8, Iss 1791, p 1791 (2020) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8111791⟩ |
| Popis: | Willaertia magna C2c Maky is a free-living amoeba that has demonstrated its ability to inhibit the intracellular multiplication of some Legionella pneumophila strains, which are pathogenic bacteria inhabiting the aquatic environment. The Amoeba, an industry involved in the treatment of microbiological risk in the water and plant protection sectors, has developed a natural biocide based on the property of W. magna to manage the proliferation of the pathogen in cooling towers. In axenic liquid medium, amoebas are usually cultivated in adhesion on culture flask. However, we implemented a liquid culture in suspension using bioreactors in order to produce large quantities of W. magna. In order to investigate the culture condition effects on W. magna, we conducted a study based on microscopic, proteomics and lipidomics analyzes. According to the culture condition, amoeba exhibited two different phenotypes. The differential proteomics study showed that amoebas seemed to promote the lipid metabolism pathway in suspension culture, whereas we observed an upregulation of the carbohydrate pathway in adherent culture. Furthermore, we observed an over-regulation of proteins related to the cytoskeleton for W. magna cells grown in adhesion. Regarding the lipid analysis, suspension and adhesion cell growth showed comparable lipid class compositions. However, the differential lipid analysis revealed differences that confirmed cell phenotype differences observed by microscopy and predicted by proteomics. Overall, this study provides us with a better insight into the biology and molecular processes of W. magna in different culture lifestyles. |
| Databáze: | OpenAIRE |
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