Campylobacter fetus Induced Proinflammatory Response in Bovine Endometrial Epithelial Cells
| Autor: | Daniel Martínez-Gómez, Estela T Méndez-Olvera, Lizeth Guadalupe Campos-Múzquiz, Monika Palacios Martínez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) lcsh:QH426-470 030106 microbiology lcsh:QR1-502 Biology 01 natural sciences Applied Microbiology and Biotechnology Microbiology lcsh:Microbiology Proinflammatory cytokine 03 medical and health sciences Immune system 010608 biotechnology pathogenicity Secretion Interleukin 8 Fetus Innate immune system pathogen-host interaction (MesH) bacterial infection General Medicine biology.organism_classification virulence lcsh:Genetics Campylobacter fetus Intracellular |
| Zdroj: | Polish Journal of Microbiology, Vol 70, Iss 1 (2021) Polish Journal of Microbiology |
| ISSN: | 2544-4646 1733-1331 |
| Popis: | Campylobacter fetus subsp. fetus is the causal agent of sporadic abortion in bovines and infertility that produces economic losses in livestock. In many infectious diseases, the immune response has an important role in limiting the invasion and proliferation of bacterial pathogens. Innate immune sensing of microorganisms is mediated by pattern-recognition receptors (PRRs) that identify pathogen-associated molecular patterns (PAMPs) and induces the secretion of several proinflammatory cytokines, like IL-1β, TNF-α, and IL-8. In this study, the expression of IL-1β, TNF-α, IL-8, and IFN-γ in bovine endometrial epithelial cells infected with C. fetus and Salmonella Typhimurium (a bacterial invasion control) was analyzed. The results showed that expression levels of IL-1β and IL-8 were high at the beginning of the infection and decreased throughout the intracellular period. Unlike in this same assay, the expression levels of IFN-γ increased through time and reached the highest peak at 4 hours post infection. In cells infected with S. Typhimurium, the results showed that IL8 expression levels were highly induced by infection but not IFN-γ. In cells infected with S. Typhimurium or C. fetus subsp. fetus, the results showed that TNF-α expression did not show any change during infection. A cytoskeleton inhibition assay was performed to determine if cytokine expression was modified by C. fetus subsp. fetus intracellular invasion. IL-1β and IL-8 expression were downregulated when an intracellular invasion was avoided. The results obtained in this study suggest that bovine endometrial epithelial cells could recognize C. fetus subsp. fetus resulting in early proinflammatory response. |
| Databáze: | OpenAIRE |
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