Differential UDP-galactose-4′-epimerase (GALE) enzymatic activity and mRNA expression in the rat mammary gland during lactation
| Autor: | Nathalie Daude, Yoon S. Shin, Laetitia Prestoz, Klaus G. Petry, I. Cournu |
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| Přispěvatelé: | University of Alberta, CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Neuroinflammation: imagerie et thérapie de la sclérose en plaques, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 1998 |
| Předmět: |
Galactose metabolism
[SDV]Life Sciences [q-bio] Mrna expression Mammary gland Biophysics Biology Biochemistry UDPglucose 4-Epimerase 03 medical and health sciences Mammary Glands Animal 0302 clinical medicine Structural Biology Lactation Genetics medicine Transcriptional regulation Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Rats Wistar Molecular Biology ComputingMilieux_MISCELLANEOUS DNA Primers 030304 developmental biology chemistry.chemical_classification 0303 health sciences Messenger RNA Enzymatic activity Base Sequence Galactosemia UDP-galactose-4′-epimerase mRNA expression RNA Cell Biology medicine.disease Rats Cell biology carbohydrates (lipids) medicine.anatomical_structure Enzyme chemistry Female 030217 neurology & neurosurgery |
| Zdroj: | FEBS Letters FEBS Letters, Wiley, 1998, 431 (3), pp.391-394. ⟨10.1016/s0014-5793(98)00796-0⟩ |
| ISSN: | 0014-5793 1873-3468 |
| Popis: | We have investigated the UDP-galactose-4′-epimerase (GALE) enzymatic activity and mRNA expression in the rat mammary gland during lactation. We report a dramatic increase in the GALE enzymatic activity correlated with an increase in the mRNA transcript expression. These results indicate a transcriptional regulation of the enzyme during lactation in the rat mammary gland. Our data are of double interest for further investigation: first, the mammary gland provides a suitable model for the characterisation of the transcriptional regulation elements of GALE which are still unknown in mammals; second, GALE expression could help to compensate UDP-galactose deficiency in classic galactosaemia. |
| Databáze: | OpenAIRE |
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