Upregulation of circular RNA circ_0001721 predicts unfavorable prognosis in osteosarcoma and facilitates cell progression via sponging miR-569 and miR-599
| Autor: | Guohua Yin, Guanghao Yu, Yanming Pan, Yanjing Zhao, Liandi Li, Ling Guo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Cell Mice Nude RM1-950 miR-599 Biology medicine.disease_cause Cell Line Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Circular RNA medicine Animals Humans Psychological repression Pharmacology Mice Inbred BALB C Osteosarcoma miR-569 RNA Circular circ_0001721 General Medicine Middle Aged Prognosis medicine.disease Xenograft Model Antitumor Assays Up-Regulation MicroRNAs 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Disease Progression Cancer research RNA Female Therapeutics. Pharmacology DNA microarray Carcinogenesis |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 109, Iss, Pp 226-232 (2019) |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2018.10.072 |
| Popis: | Osteosarcoma (OS) is the most frequent bone malignancy in human. Growing evidence suggests that circular RNAs (circRNAs) play a significant role in regulating tumorigenesis and progression. Previously, circ_0001721 was identified upregulated in OS tissues screened by circRNA microarrays. In the current study, circ_0001721 expression in OS tissue samples and cells were measured by qRT-PCR. Its clinical value was also explored. For the part of functional assays, CCK-8, clone-forming, flow cytometric, Transwell, xenograft assays were performed. Dual luciferase reporter assays were used to examine the mechanism of circ_0001721. We found that circ_0001721 was enhanced in OS tissue samples and cell lines and the overexpression of circ_0001721 is closely related to clinical severity. In addition, circ_0001721 may be used as a prognostic indicator for OS patients. What's more, loss-of-function and gain-of-function assays demonstrated circ_0001721 facilitates cell progression in OS. Circ_0001721 could sponge miR-569 and miR-599. The oncogenic properties of circ_0001721 is partly attributed to its repression on miR-569 and miR-599. Collectively, the present data reveal the participation of circ_0001721 in tumorigenesis of OS cells, and may indicate a novel therapeutic target. |
| Databáze: | OpenAIRE |
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