Characterization of the structural gene encoding a copper-containing nitrite reductase and homology of this gene to DNA of other denitrifiers
| Autor: | Marcos R. Fries, S G Bezborodnikov, Rick W. Ye, B A Averill, James M. Tiedje |
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| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Nitrite Reductases Molecular Sequence Data Biology Applied Microbiology and Biotechnology Bacterial Proteins Species Specificity Pseudomonas Sequence Homology Nucleic Acid Amino Acid Sequence Peptide sequence Gene Nitrites Southern blot Base Sequence Ecology Structural gene Nucleic acid sequence biology.organism_classification Nitrite reductase Molecular biology Pseudomonas stutzeri Biochemistry Genes Bacterial Molecular probe Copper Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 59:250-254 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.59.1.250-254.1993 |
| Popis: | A copper-containing nitrite reductase gene (nirU) from Pseudomonas sp. strain G-179 was found in a 1.9-kb EcoRI-BamHI DNA fragment. The coding region contained information for a polypeptide of 379 amino acids. The encoded protein had 78% identity in amino acid sequence to the nitrite reductase purified from Achromobacter cycloclastes. The ligands for type 1 copper- and type 2 copper-binding sites found in A. cycloclastes were also found in Pseudomonas sp. strain G-179, suggesting that these binding sites are conserved. Upstream from the promoter, two putative fnr boxes were found, suggesting that an FNR-like protein may be involved in regulation of the nitrite reductase gene under anaerobic conditions. When the 1.9-kb clone was used to probe Southern blots for similar sequences in DNAs from different denitrifiers, hybridization bands were seen for 15 of 16 denitrifiers known to have nitrite reductase containing copper. Except for Pseudomonas stutzeri JM300, all denitrifiers tested that have nitrite reductases containing heme c,d1 showed no or weak hybridization to this probe. Thus, this structural gene may be useful as a probe to detect denitrifiers with copper-containing nitrite reductases. |
| Databáze: | OpenAIRE |
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