MiR-221/SIRT1/Nrf2 signal axis regulates high glucose induced apoptosis in human retinal microvascular endothelial cells
| Autor: | Ting Cao, Tao He, Li Wu, Hong-Mei Zheng, Bin Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
NF-E2-Related Factor 2 Apoptosis environment and public health Nrf2 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine SIRT1 Western blot lcsh:Ophthalmology Sirtuin 1 Diabetic retinopathy Diabetes mellitus hRMEC Medicine Humans MTT assay Messenger RNA Reporter gene medicine.diagnostic_test business.industry Endothelial Cells Retinal General Medicine medicine.disease miR-221 Ophthalmology MicroRNAs 030104 developmental biology Glucose chemistry lcsh:RE1-994 030220 oncology & carcinogenesis Cancer research business Research Article |
| Zdroj: | BMC Ophthalmology BMC Ophthalmology, Vol 20, Iss 1, Pp 1-10 (2020) |
| ISSN: | 1471-2415 |
| Popis: | Background Diabetic retinopathy (DR) is a serious symptom associated with diabetes and could cause much suffer to patients. MiR-221, SIRT1 and Nrf2 were associated with apoptosis and proliferation and their expression were altered in DR patients. However, their roles and regulatory mechanisms in human retinal microvascular endothelial cells (hRMEC) were not clear. Methods Expression of mRNA was detected by qRT-PCR. Protein expression was detected by Western blot. Interaction between miR-221 and SIRT1 was predicted by bioinformatics analysis and validated by dual-luciferase reporter assay. We analyzed the viability and apoptosis of hRMEC by MTT assay and FACS assay, respectively. Results High glucose (HG) treatment enhanced expression of miR-221 and inhibited expression of SIRT1 and Nrf2. MiR-221 overexpression promoted apoptosis under HG condition. Moreover, miR-221 directly interacted with mRNA of SIRT1 and inhibited SIRT1 expression in hRMEC, through which miR-221 inhibited Nrf2 pathway and induced apoptosis of hRMEC. Conclusion Our data demonstrated that miR-221/SIRT1/Nrf2 signal axis could promote apoptosis in hRMEC under HG conditions. This finding could provide theoretical support for future studies and may contribute to development of new treatment options to retard the process of DR development. |
| Databáze: | OpenAIRE |
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