Lycopene phytocomplex, but not pure lycopene, is able to trigger apoptosis and improve the efficacy of photodynamic therapy in HL60 human leukemia cells
| Autor: | Anna Ettorre, Anna Di Stefano, Simona Frosali, Marco Andreassi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
HL60
medicine.medical_treatment alpha-Tocopherol Apoptosis HL-60 Cells Photodynamic therapy Phosphatidylserines Biology General Biochemistry Genetics and Molecular Biology Membrane Potentials chemistry.chemical_compound Lycopene medicine Humans Cytotoxic T cell Photosensitizer chemistry.chemical_classification Reactive oxygen species Leukemia Photosensitizing Agents Glutathione Ascorbic acid Carotenoids Mitochondria Photochemotherapy Biochemistry chemistry Cancer research Reactive Oxygen Species |
| Zdroj: | Experimental Biology and Medicine. 235:1114-1125 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | We compared the ability of pure lycopene (Lyco) versus lycopene phytocomplex (LycoC) to induce apoptosis in vitro. We found that LycoC, but not Lyco, was able to trigger apoptosis in HL60 cells, as documented by subdiploid DNA content and phosphatidylserine exposure. LycoC-induced apoptosis was associated with reactive oxygen species (ROS) generation and loss of mitochondrial transmembrane potential, suggesting that LycoC triggered apoptosis via a mitochondrial pathway. We also verified the redox state of cells by measuring glutathione (GSH) content, but only a small percentage of cells showed GSH depletion, suggesting that the loss of GSH may be a secondary consequence of ROS generation. Moreover, LycoC pretreatment effectively increased apoptosis induced by photodynamic therapy (PDT), a mode of cancer treatment using a photosensitizer and visible light. LycoC pretreatment was even more potent in improving PDT than pretreatment with ascorbic acid or alpha-tocopherol (or the two combined). Our results demonstrate that LycoC has a stronger cytotoxic effect than Lyco and is a better source of agents able to trigger apoptosis in HL60 cells and improve the efficacy of PDT in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|