IL-6 and IL-20 as potential markers for vitality of skin contusion
| Autor: | Ming-Yuan Ye, Xiao-Li Xie, Kai-Kai Zhang, Ye Xue, Hao-Pin Lyu, Di Xu, Jie-Tao He, Hong-Yan Huang, Qi Wang, Jin-Cen Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Adolescent Contusions Human skin Real-Time Polymerase Chain Reaction Vitality Pathology and Forensic Medicine law.invention Young Adult 03 medical and health sciences 0302 clinical medicine Interleukin 20 law Animals Humans Medicine RNA Messenger 030216 legal & forensic medicine Interleukin 6 Forensic Pathology Polymerase chain reaction Skin Mice Inbred BALB C Messenger RNA integumentary system biology Interleukin-6 business.industry Interleukins General Medicine Middle Aged Reverse transcriptase 030104 developmental biology Postmortem contusion Postmortem Changes biology.protein Female business Law Biomarkers |
| Zdroj: | Journal of Forensic and Legal Medicine. 59:8-12 |
| ISSN: | 1752-928X |
| Popis: | The detection of vitality of wounds is very important in forensic practice. This study is performed using quantitative real-time reverse transcriptase polymerase chain reaction (RT-qPCR) in both mouse and human skin wounds for the application of IL-6 and IL-20 in order to differentiate intravital wounds from postmortem wounds. RT-qPCR analysis of contused mouse skin showed that increased IL-6 and IL-20 mRNA levels were found in comparison to intact skin tissues. The increased mRNA expressions of IL-6 and IL-20 were observed until 72 h after death in contused mouse skin, whereas there were no marked changes in these two cytokines in the postmortem contusion group. The alterations of IL-6 and IL-20 can also be detected in human skin wound samples. These finding suggest that mRNA levels of IL-6 and IL-20 might be used as potential markers for vital reaction. |
| Databáze: | OpenAIRE |
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