The α-1,6-mannosyltransferase VdOCH1 plays a major role in microsclerotium formation and virulence in the soil-borne pathogen Verticillium dahliae
| Autor: | Jianfeng Yang, Liru Kang, Jun Zhao, Yuan-Yuan Zhang, Jian Zhang, Addrah Mandela EloRM, Zhou Hongyou |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Mutant Virulence Verticillium Mannosyltransferases 01 natural sciences Microbiology Fungal Proteins 03 medical and health sciences Cell Wall Genetics Verticillium dahliae Soil Microbiology Ecology Evolution Behavior and Systematics Plant Diseases Sequence Deletion 030304 developmental biology 0303 health sciences biology Genetic Complementation Test Wild type Agrobacterium tumefaciens Spores Fungal biology.organism_classification Sunflower Complementation Mutagenesis Insertional Infectious Diseases Helianthus Verticillium wilt 010606 plant biology & botany |
| Zdroj: | Fungal Biology. 123:539-546 |
| ISSN: | 1878-6146 |
| DOI: | 10.1016/j.funbio.2019.05.007 |
| Popis: | Sunflower yellow wilt is a widespread and destructive disease caused by the soil-borne pathogen Verticillium dahliae (V. dahliae). To better understand the pathogenesis mechanism of V. dahliae in sunflower, T-DNA insertion library was generated via Agrobacterium tumefaciens mediated transformation system (ATMT). Eight hundred positive transformants were obtained. Transformants varied in colony morphology, growth rate, conidia production and pathogenicity in sunflower compared to the wild type strain. A mutant, named VdGn3-L2, was chosen for further analysis based on its deprivation on microsclerotia formation. The flanking sequence of T-DNA insertion site of VdGn3-L2 was identified via hiTAIL-PCR, and the interrupted gene encoded an initiation-specific α-1, 6-mannosyltransferase, named as VdOCH1. The deletion mutant ΔVdOCH1 was impaired in certain characteristics such as fungal growth, conidia production, and microsclerotia formation. Also, ΔVdOCH1 mutants were more sensitive to the cell wall perturbing reagents, such as SDS and Congo red, lost their penetration ability through cellophane membrane, and exhibited dramatically decreased pathogenicity to sunflower. The impaired phenotypes could be restored to the wild type level by complementation of the deletion mutant with full-length VdOCH1 gene. In conclusion, VdOCH1, encoded α-1,6-mannosyltransferase, manipulating the biological characteristics, microsclerotia formation and pathogenic ability of V. dahliae in sunflower. |
| Databáze: | OpenAIRE |
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