Excess body weight and age associated with the carriage of fluoroquinolone and third-generation cephalosporin resistance genes in commensal Escherichia coli from a cohort of urban Vietnamese children
| Autor: | Ha Thanh Tuyen, Nguyen Van Vinh Chau, Le Thi Quynh Nhi, Tran Thi Thuy Tien, Tran Do Hoang Nhu, Hoang Thi Diem Tuyet, Guy E. Thwaites, Stephen Baker, Pham Thanh Duy, Nguyen Thi Thanh Nhan, Pham Duc Trung, Lu Lan Vi, Phung Khanh Lam |
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| Přispěvatelé: | Baker, Stephen [0000-0003-1308-5755], Apollo - University of Cambridge Repository |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) medicine.medical_specialty 030106 microbiology Microbial Sensitivity Tests EBSL Microbiology Feces 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance Ciprofloxacin Internal medicine Drug Resistance Bacterial Escherichia coli medicine Humans antimicrobial resistance Prospective Studies Escherichia coli Infections co-selection business.industry Body Weight Age Factors cohort General Medicine Odds ratio medicine.disease Obesity third generation cephalosporins Anti-Bacterial Agents Carriage Vietnam chemistry Child Preschool Carrier State Cohort Ceftriaxone Female MacConkey agar business plasmid mediated quinolone resistance Fluoroquinolones medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 67:1457-1466 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.000820 |
| Popis: | Purpose. Antimicrobial-resistant bacterial infections in low- and middle-income countries (LMICs) are a well-established global health issue. We aimed to assess the prevalence of and epidemiological factors associated with the carriage of ciprofloxacin- and ceftriaxone-resistant Escherichia coli and associated resistance genes in a cohort of 498 healthy children residing in urban Vietnam. Methodology. We cultured rectal swabs onto MacConkey agar supplemented with resistant concentrations of ciprofloxacin and ceftriaxone. Additionally, we screened meta-E. coli populations by conventional PCR to detect plasmid-mediated quinolone resistance (PMQR)- and extended-spectrum β-lactamase (ESBL)-encoding genes. We measured the associations between phenotypic/genotypic resistance and demographic characteristics using logistic regression. Results/key findings. Ciprofloxacin- and ceftriaxone-resistant E. coli were cultured from the faecal samples of 67.7 % (337/498) and 80.3 % (400/498) of children, respectively. The prevalence of any associated resistance marker in the individual samples was 86.7 % (432/498) for PMQR genes and 90.6 % (451/498) for β-lactamase genes. Overweight children were significantly more likely to carry qnr genes than children with lower weight-for-height z-scores [odds ratios (OR): 1.24; 95 % confidence interval (CI): 10.5–1.48 for each unit increase in weight for height; P=0.01]. Additionally, younger children were significantly more likely to carry ESBL CTX-M genes than older children (OR: 0.97, 95 % CI: 0.94–0.99 for each additional year, P=0.01). Conclusion. The carriage of genotypic and phenotypic antimicrobial resistance is highly prevalent among E. coli in healthy children in the community in Vietnam. Future investigations on the carriage of antimicrobial resistant organisms in LMICs should focus on the progression of carriage from birth and structure of the microbiome in obesity. |
| Databáze: | OpenAIRE |
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