Evaluation of antibody immunochromatography testing for diagnosis of current Helicobacter pylori infection
| Autor: | Nashwan N. Alkhawlani, Sammer M.S. Alshuaibi, Jamil M.A.S. Obaid, Ayoob N.N. Ayoon, Omar N.M. Almurisy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Abdominal pain Medicine (General) Clinical Biochemistry Population Gastroenterology Sensitivity R5-920 Antigen Internal medicine medicine education QD1-999 education.field_of_study Radiological and Ultrasound Technology biology medicine.diagnostic_test business.industry Gastric ulcer Heartburn Complete blood count Serum antibody test Diarrhea Chemistry biology.protein Specificity Antibody medicine.symptom Gastritis business H. pylori Stool antigen test |
| Zdroj: | Practical Laboratory Medicine, Vol 26, Iss, Pp e00245-(2021) |
| ISSN: | 2352-5517 |
| Popis: | Background Helicobacter pylori infection represents a major gastrointestinal complaint associated with gastritis, gastric ulcer and stomach tumors. It is present in 90 % of developing countries population. H. pylori diagnosis in these countries, where resources are limited, is accomplished with simple non-invasive tests such as stool antigen and serum antibody tests. The aim of this study was to evaluate the serum antibody test in the diagnosis of current H. pylori infection. Subject and methods A total of 117 patients were included in this prospective diagnosis accuracy testing study, who clinically presented with dyspepsia, heartburn, abdominal pain, diarrhea, or halitosis. A stool sample was collected from each patient and tested for H. pylori antigen using immunochromatographic method. Blood sample was also collected, half of which was EDTA-sampled and analyzed for complete blood count, while the remaining half was left to clot, the separated serum was tested for antibodies against H. pylori with immunochromatographic cassette. Results About 35 % of sixty six patients who were positive for stool antigen test gave a negative for serum antibodies test. Meanwhile, the non-consistent results within 51 negative stool antigen test patients was exhibited by 47 % of them. The discrepancies were not affected by age or disease duration. The calculated sensitivity, specificity, positive predictive value and negative predictive values were 50 %, 65 %, 65 % and 50 % respectively. Conclusion The serum antibody test is not reliable in the diagnosis of current H. pylori infection. In developing countries, with limited facilities and primary care units, stool antigen test diagnosis is useful for diagnosis. |
| Databáze: | OpenAIRE |
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