Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines
| Autor: | Andrzej Wilk, Nagaradona Harindranath, Serge L. Beaucage, Tomas Kempe, Jila H. Boal, Edward E. Max |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Guanine
Molecular Sequence Data Biology Cleavage (embryo) Gas phase Cytosine Methylamines chemistry.chemical_compound Ammonia Organophosphorus Compounds Controlled pore glass Pressure Genetics Base Sequence Methylamine Adenine Combinatorial chemistry Oligodeoxyribonucleotides chemistry Biochemistry Gases Glass Research Article |
| Zdroj: | Nucleic Acids Research. 24:3115-3117 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/24.15.3115 |
| Popis: | A novel method for the deprotection of oligodeoxyribonucleotides has been developed. Gaseous amines such as ammonia or methylamine were employed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides having a (tert-butyl)phenoxyacetyl group for the protection of the exocyclic amino function of cytosine, adenine and guanine were released from controlled-pore glass supports and fully deprotected by ammonia or methylamine under gas phase conditions, at room temperature, within 35 or 2 min respectively. |
| Databáze: | OpenAIRE |
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