Overexpression of activin-beta A subunit mRNA is associated with decreased activin type II receptor mRNA levels in the testes of alpha-inhibin deficient mice
| Autor: | V.L. Trudeau, R. J. G. Haché, M.M. Matzuk, L. P. Renaud |
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| Rok vydání: | 1994 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Macromolecular Substances Activin Receptors Biophysics Hypothalamus Gene Expression Biology Protein Serine-Threonine Kinases Biochemistry Paracrine signalling Mice Internal medicine Gene expression Testis medicine Animals Inhibins Receptors Growth Factor Northern blot RNA Messenger Receptor Molecular Biology Activin type 2 receptors Amino Acid Isomerases Messenger RNA Endodeoxyribonucleases Cell Biology Activin receptor RNA Probes Peptidylprolyl Isomerase Blotting Northern Molecular biology Mice Mutant Strains Activins Blot Endocrinology Carrier Proteins hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biochemical and biophysical research communications. 203(1) |
| ISSN: | 0006-291X |
| Popis: | Activins and inhibins are polypeptides of the transforming growth factor-beta family that participate in differentiation and growth of diverse cell types, and are involved in endocrine/paracrine regulation of the hypothalamo-pituitary-gonadal axis. Mice with alpha-inhibin subunit gene deletion develop large testicular tumors. In these animals, a 200-fold increase in testicular expression of activin beta A subunit mRNA was detected using S1-nuclease protection analysis. Northern blot analysis demonstrated that a predominant mRNA form of approximately 6.5 kb and a second minor form of 4.5 kb were overexpressed in the testes of the alpha-inhibin deficient animals. Testicular expression of the type II activin receptor was decreased 3-fold in these mice. In contrast, hypothalamic beta A and type II activin receptor mRNA levels remained unaltered. alpha-Inhibin may play a role to suppress the expression of beta A mRNA in the mammalian testes. These results demonstrate that increased expression of activin is accompanied by a tissue specific reduction in the expression of its own receptor mRNA in vivo. |
| Databáze: | OpenAIRE |
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