Simple and Sensitive Method for Pyrroloquinoline Quinone (PQQ) Analysis in Various Foods Using Liquid Chromatography/Electrospray-Ionization Tandem Mass Spectrometry
| Autor: | Natsumi Noji, Shigeo Yoshida, Tamizi Sugiyama, Tadao Asami, Masafumi Tsujimoto, Katsuhiko Taguchi, Nobutaka Kitahata, Takemichi Nakamura, Toshiaki Kudo |
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| Rok vydání: | 2007 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Chromatography Chemistry Selected reaction monitoring Fresh weight PQQ Cofactor General Chemistry Tandem mass spectrometry Sensitivity and Specificity High-performance liquid chromatography Highly sensitive chemistry.chemical_compound Liquid chromatography electrospray ionization tandem mass spectrometry Pyrroloquinoline quinone Plants Edible General Agricultural and Biological Sciences Quantitative analysis (chemistry) Chromatography High Pressure Liquid Food Analysis |
| Zdroj: | Journal of Agricultural and Food Chemistry. 55:7258-7263 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf070483r |
| Popis: | Pyrroloquinoline quinone (PQQ) is believed to be an important factor for mammalian growth and development and has, therefore, been declared a vitamin by some researchers. However, this issue remains controversial, and from a nutritional viewpoint, accurate determination of PQQ levels in a variety of foods is very important. Here, we describe a simple, highly sensitive, and highly selective method for quantitative analysis of PQQ. Liquid foods or aqueous extracts of solid foods were analyzed using high-performance liquid chromatography (HPLC) combined with electrospray-ionization (ESI) tandem mass spectrometry (MS/MS). (15)N-labeled PQQ was added to the samples as an internal standard. Quantitative analyses of PQQ were performed by multiple reaction monitoring (MRM) with LC/MS/MS. Free PQQ was detected in almost all food samples in the range 0.19-7.02 ng per g fresh weight (for solid foods) or per mL (liquid foods). This method will enable the rapid and simple determination of PQQ levels in many samples. |
| Databáze: | OpenAIRE |
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