Control of apterous by vestigial drives indirect flight muscle development in drosophila

Autor: Fred Bernard, J.F Ferveur, R. Cossard, J Silber, M Gullaud, A Lalouette, A Zider, A.Y Jeantet
Přispěvatelé: Développement et Communication Chimique chez les Insectes ( DCCI ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Delon, Viviane
Rok vydání: 2003
Předmět:
Male
Nerve Tissue Proteins/genetics/metabolism
Muscle Fibers
Skeletal
Mutant
Transcription Factors/genetics/*metabolism
medicine.disease_cause
Myoblasts
Twist transcription factor
Myoblasts/physiology
Drosophila Proteins
Wings
Animal
Developmental
Cells
Cultured
Regulator gene
Regulation of gene expression
Wing/growth & development/physiology
Mutation
Cultured
Muscles
Gene Expression Regulation
Developmental
Nuclear Proteins
Drosophila Proteins/genetics/*metabolism
Anatomy
Muscle degeneration
Cell biology
twist
Drosophila
cut
Muscles/metabolism/pathology/*physiology
Indirect flight muscles
Cells
LIM-Homeodomain Proteins
Muscle Fibers/pathology/physiology
Nerve Tissue Proteins
Biology
vestigial
Nuclear Proteins/genetics/*metabolism
medicine
Homeodomain Proteins/genetics/*metabolism
Animals
Drosophila/*growth & development
Molecular Biology
Homeodomain Proteins
Twist-Related Protein 1
Cell Biology
biology.organism_classification
apterous
Twist Transcription Factor
Gene Expression Regulation
Ectopic expression
Transcription Factors
Developmental Biology
Zdroj: Developmental Biology
Developmental Biology, Elsevier, 2003, 260 (2), pp.391-403
ISSN: 0012-1606
1095-564X
DOI: 10.1016/s0012-1606(03)00255-0
Popis: 0012-1606 (Print) Journal Article Research Support, Non-U.S. Gov't; Drosophila thoracic muscles are comprised of both direct flight muscles (DFMs) and indirect flight muscles (IFMs). The IFMs can be further subdivided into dorsolongitudinal muscles (DLMs) and dorsoventral muscles (DVMs). The correct patterning of each category of muscles requires the coordination of specific executive regulatory programs. DFM development requires key regulatory genes such as cut (ct) and apterous (ap), whereas IFM development requires vestigial (vg). Using a new vg(null) mutant, we report that a total absence of vg leads to DLM degeneration through an apoptotic process and to a total absence of DVMs in the adult. We show that vg and scalloped (sd), the only known VG transcriptional coactivator, are coexpressed during IFM development. Moreover, we observed an ectopic expression of ct and ap, two markers of DFM development, in developing IFMs of vg(null) pupae. In addition, in vg(null) adult flies, degenerating DLMs express twist (twi) ectopically. We provide evidence that ap ectopic expression can induce per se ectopic twi expression and muscle degeneration. All these data seem to indicate that, in the absence of vg, the IFM developmental program switches into the DFM developmental program. Moreover, we were able to rescue the muscle phenotype of vg(null) flies by using the activity of ap promoter to drive VG expression. Thus, vg appears to be a key regulatory gene of IFM development.
Databáze: OpenAIRE
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