Comparison of a Commercial H1N1 Enzyme-Linked Immunosorbent Assay and Hemagglutination Inhibition Test in Detecting Serum Antibody against Swine Influenza Viruses
| Autor: | Gene Erickson, Rick W. Swalla, Bruce H. Janke, Kyoung-Jin Yoon |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
0301 basic medicine
Swine viruses 030106 microbiology Population Enzyme-Linked Immunosorbent Assay Antibodies Viral medicine.disease_cause Virus Serology 03 medical and health sciences Influenza A Virus H1N1 Subtype Orthomyxoviridae Infections Antibody Specificity Influenza A virus medicine Animals education Swine Diseases education.field_of_study Hemagglutination assay General Veterinary biology Inoculation Antibody titer virus diseases Hemagglutination Inhibition Tests Virology 030104 developmental biology Immunoglobulin M biology.protein Reagent Kits Diagnostic Antibody |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 16:197-201 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/104063870401600304 |
| Popis: | Recently a commercial enzyme-linked immunosorbent assay (ELISA) kit for detecting antibody against H1N1 swine influenza virus (SIV) has been made available to diagnosticians and veterinary practitioners. Because the hemagglutination inhibition (HI) test has been considered the standard test for SIV serology, diagnostic performance of the new ELISA was evaluated using positive ( n = 60) and negative ( n = 188) serum samples from young pigs with known status of SIV infection and compared with that of the HI test. Both ELISA and HI test identified all negative animals correctly. None of the serum samples ( n = 64) from pigs inoculated with H3N2 SIV was positive by ELISA for SIV antibody. The H1N1 SIV antibody detectable by ELISA appears to develop more slowly in comparison with antibody detectable by HI test. Although antibody was detected by HI test in all inoculated animals ( n = 20) by day 7 postinoculation (PI), antibody was detected by ELISA in 0%, 75%, and 100% of the inoculated animals on days 7, 14, and 28 PI, respectively. Discrepancy in test results between the 2 serologic tests appeared to be because of differences in antibody isotypes detected by each test. Enzyme-linked immunosorbent assay mainly detected IgG antibody, whereas the HI test detects IgM antibody very efficiently as well as IgG antibody. Collectively, the commercial ELISA is highly specific for antibody to H1N1 SIV but may not identify positive animals at the early stage of infection as effectively as the HI test, particularly when SIV is introduced to a naïve swine population. |
| Databáze: | OpenAIRE |
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