Interplay between the β′ Clamp and the β′ Jaw Domains during DNA Opening by the Bacterial RNA Polymerase at σ54-dependent Promoters
| Autor: | Konstantin Severinov, Martin Buck, Siva R. Wigneshweraraj, Dhruti Savalia |
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| Rok vydání: | 2006 |
| Předmět: |
Models
Molecular Transcription Genetic Specificity factor Repressor Biology chemistry.chemical_compound Structural Biology Sigma factor RNA polymerase Escherichia coli Promoter Regions Genetic Molecular Biology Polymerase DNA clamp Heparin DNA DNA-Directed RNA Polymerases Molecular biology Protein Structure Tertiary Cell biology Repressor Proteins RNA Polymerase Sigma 54 chemistry Mutation biology.protein Nucleic Acid Conformation bacteria Transcription Initiation Site |
| Zdroj: | Journal of Molecular Biology. 359:1182-1195 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/j.jmb.2006.04.063 |
| Popis: | The bacterial RNA polymerase (RNAP) is a multi-subunit, structurally flexible, complex molecular machine, in which activities associated with DNA opening for transcription-competent open promoter complex (OC) formation reside in the catalytic beta and beta' subunits and the dissociable sigma subunit. OC formation is a multi-step process that involves several structurally conserved mobile modules of beta, beta', and sigma. Here, we present evidence that two flexible modules of beta', the beta' jaw and the beta' clamp and a conserved regulatory Region I domain of sigma(54), jointly contribute to the maintenance of stable DNA strand separation around the trancription start site in OCs formed at sigma(54)-dependent promoters. Clearly, regulated interplay between the mobile modules of the beta' and the sigma subunits of the RNAP appears to be necessary for stable OC formation. |
| Databáze: | OpenAIRE |
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