BDMC protects AD in vitro via AMPK and SIRT1
| Autor: | Duanqun He, Gang Wang, Yihui Li, Zijian Xiao, Guijuan Zhou, Ming Xie, Chenlin Xu, Heng Wu, Yunqian Chang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
AMPK
Programmed cell death General Neuroscience Neurotoxicity Alzheimer’s disease (AD) bisdemethoxycurcumin (BDMC) Neurosciences. Biological psychiatry. Neuropsychiatry Pharmacology medicine.disease Neuroprotection chemistry.chemical_compound SIRT1 chemistry Apoptosis Bisdemethoxycurcumin Curcumin medicine Viability assay RC321-571 Research Article |
| Zdroj: | Translational Neuroscience Translational Neuroscience, Vol 11, Iss 1, Pp 319-327 (2020) |
| ISSN: | 2081-6936 2081-3856 |
| Popis: | Background Alzheimer’s disease (AD) is a common neurodegenerative disorder without any satisfactory therapeutic approaches. AD is mainly characterized by the deposition of β-amyloid protein (Aβ) and extensive neuronal cell death. Curcumin, with anti-oxidative stress (OS) and cell apoptosis properties, plays essential roles in AD. However, whether bisdemethoxycurcumin (BDMC), a derivative of curcumin, can exert a neuroprotective effect in AD remains to be elucidated. Methods In this study, SK-N-SH cells were used to establish an in vitro model to investigate the effects of BDMC on the Aβ1–42-induced neurotoxicity. SK-N-SH cells were pretreated with BDMC and with or without compound C and EX527 for 30 min after co-incubation with rotenone for 24 h. Subsequently, western blotting, cell viability assay and SOD and GSH activity measurement were performed. Results BDMC increased the cell survival, anti-OS ability, AMPK phosphorylation levels and SIRT1 in SK-N-SH cells treated with Aβ1–42. However, after treatment with compound C, an AMPK inhibitor, and EX527, an SIRT1inhibitor, the neuroprotective roles of BDMC on SK-N-SH cells treated with Aβ1–42 were inhibited. Conclusion These results suggest that BDMC exerts a neuroprotective role on SK-N-SH cells in vitro via AMPK/SIRT1 signaling, laying the foundation for the application of BDMC in the treatment of neurodegenerative diseases related to AMPK/SIRT1 signaling. |
| Databáze: | OpenAIRE |
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