Impaired Thrombin Generation in β2-Glycoprotein I Null Mice
| Autor: | Yonghua Sheng, Stephen W. Reddel, Herbert Herzog, Ying Xia Wang, Tim Brighton, Malcolm P. France, Sarah A. Robertson, Steven A. Krilis |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Blood Platelets
Male Heterozygote Time Factors Offspring Immunoblotting Mice Transgenic medicine.disease_cause Biochemistry Mice Thrombin medicine Animals Beta 2-Glycoprotein I Platelet Blood Coagulation Molecular Biology Alleles Crosses Genetic Glycoproteins Recombination Genetic Mutation Models Genetic biology Heterozygote advantage Cell Biology Blotting Northern Blood Coagulation Factors Mice Inbred C57BL Fertility Phenotype Coagulation beta 2-Glycoprotein I Immunology biology.protein Electrophoresis Polyacrylamide Gel Female Antibody medicine.drug |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m010990200 |
| Popis: | Autoimmune antibodies to beta(2)-glycoprotein I (beta2GPI) have been proposed to be clinically relevant because of their strong association with thrombosis, miscarriage, and thrombocytopenia. By using a homologous recombination approach, beta2GPI-null mice were generated to begin to understand the physiologic and pathologic role of this prominent plasma protein in mammals. When beta2GPI heterozygotes on a 129/Sv/C57BL/6 mixed genetic background were intercrossed, only 8.9% of the resulting 336 offspring possessed both disrupted alleles. These data suggest that beta2GPI plays a beneficial role in implantation and/or fetal development in at least some mouse strains. Although those beta2GPI-null mice that were born appeared to be relatively normal anatomically and histologically, subsequent analysis revealed that they possessed an impaired in vitro ability to generate thrombin relative to wild type mice. Thus, beta2GPI also appears to play an important role in thrombin-mediated coagulation. |
| Databáze: | OpenAIRE |
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