Strain Differences in Responsiveness to Repeated Restraint Stress Affect Remote Contextual Fear Memory and Blood Transcriptomics
| Autor: | Eva E. Redei, Seung Ho Jung, Stephanie A. Gacek, Katherine J. Przybyl, Sarah Kim, Candice Hatcher-Solis, Matthew J. Schipma, Sophia T. Jenz, Jeanie K. Meckes, Stephanie L. Wert, Wendy Luo, Ryan Jankord, Patrick H. Lim |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Elevated plus maze Memory Long-Term Strain (chemistry) General Neuroscience Fear Contextual fear Biology Affect (psychology) Rats Inbred WKY Rats Transcriptome 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Memory Stress (linguistics) Animals Conditioning Restraint stress Neuroscience Stress Psychological 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience. 444:76-91 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2020.07.052 |
| Popis: | The role of stress in altering fear memory is not well understood. Since individual variations in stress reactivity exist, and stress alters fear memory, exposing individuals with differing stress-reactivity to repeated stress would affect their fear memory to various degrees. We explored this question using the average stress-reactive Fisher 344 (F344) rat strain and the Wistar-Kyoto (WKY) strain with its heightened stress-reactivity. Male F344 and WKY rats were exposed to the contextual fear conditioning (CFC) paradigm and then chronic restraint stress (CRS) or no stress (NS) was administered for two weeks before a second CFC. Both recent and reinstated fear memory were greater in F344s than WKYs, regardless of the stress status. In contrast, remote memory was attenuated only in F344s after CRS. In determining whether this strain-specific response to CRS was mirrored by transcriptomic changes in the blood, RNA sequencing was carried out. Overlapping differentially expressed genes (DEGs) between NS and CRS in the blood of F344 and WKY suggest a convergence of stress-related molecular mechanisms, independent of stress-reactivity. In contrast, DEGs unique to the F344 and the WKY stress responses are divergent in their functionality and networks, beyond that of strain differences in their non-stressed state. These results suggest that in some individuals chronic or repeated stress, different from the original fear memory-provoking stress, can attenuate prior fear memory. Furthermore, the novel blood DEGs can report on the general state of stress of the individual, or can be associated with individual variation in stress-responsiveness. |
| Databáze: | OpenAIRE |
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