Residential exposure to radon and levels of histone γH2AX and DNA damage in peripheral blood lymphocytes of residents of Kowary city regions (Poland)
| Autor: | Maciej Stępnik, Jerzy Olszewski, Katarzyna Walczak, M. Brodecki, Piotr Politański, Katarzyna Domeradzka-Gajda, Marek Zmyślony, K. Kowalczyk |
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| Rok vydání: | 2020 |
| Předmět: |
Environmental Engineering
DNA damage Health Toxicology and Mutagenesis 0208 environmental biotechnology chemistry.chemical_element Radon 02 engineering and technology 010501 environmental sciences 01 natural sciences Peripheral blood mononuclear cell Histones chemistry.chemical_compound Humans Environmental Chemistry Lymphocytes 0105 earth and related environmental sciences Exposure assessment biology Public Health Environmental and Occupational Health Environmental Exposure General Medicine General Chemistry Pollution Molecular biology 020801 environmental engineering Staining Comet assay Histone chemistry Air Pollutants Radioactive biology.protein Comet Assay Poland DNA DNA Damage |
| Zdroj: | Chemosphere. 247:125748 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2019.125748 |
| Popis: | Introduction Radon-induced biological effects have been studied mainly through epidemiological investigations, and well-controlled in vitro and in vivo experiments. To provide data explaining radon exposure-induced harmful effects in natural environment, exposure assessment under these conditions is needed. The objective of the study was to examine the level of genetic damage assessed with biomarkers of DNA single- and double-strand breaks (SSBs and DSBs) in peripheral blood mononuclear cells obtained from individuals continuously exposed to Rn in homes. Naturally elevated Rn concentrations in homes can be found in the South of Poland, in Kowary city. Methods Measurements of expression of phosphorylated histone γH2AX was used as a marker of DNA double strand breaks. To detect DNA single and double-strand breaks and alkali labile sites, the alkaline comet assay was used. Oxidative damage of DNA was evaluated by formamidopyrimidyne (FPG)-modified comet assay. The blood was collected from 94 volunteers living in Kowary. Subjects were grouped according to their status of living in radon concentration ≥100 Bq/m3 (n = 67), and Results The statistically significant differences in levels of DNA damage in peripheral lymphocytes assessed with comet assay were found to be associated with levels of radon exposure in indoor air (p = 0.034). DNA damage in the comet assay was significantly correlated with DNA damage assessed with γH2AX staining. Conclusions Results of the present study indicate the suitability of alkaline comet assay for the detection of DNA damage in peripheral blood lymphocytes of people environmentally exposed to radon. |
| Databáze: | OpenAIRE |
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