Evaluation of piperine against cancer stem cells (CSCs) of hepatocellular carcinoma: Insights into epithelial-mesenchymal transition (EMT)
| Autor: | Siddharth J. Modi, Satish Y. Gabhe, Vithal M. Kulkarni, Anshuly Tiwari |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Carcinoma
Hepatocellular Epithelial-Mesenchymal Transition Polyunsaturated Alkamides Vimentin Antineoplastic Agents Molecular Dynamics Simulation 01 natural sciences Biochemistry chemistry.chemical_compound Structure-Activity Relationship Alkaloids Piperidines Cancer stem cell Drug Discovery Tumor Cells Cultured Humans Epithelial–mesenchymal transition Benzodioxoles Molecular Biology biology Dose-Response Relationship Drug Molecular Structure 010405 organic chemistry Chemistry Organic Chemistry Mesenchymal stem cell CD44 Liver Neoplasms Cell cycle Recurrent Hepatocellular Carcinoma 0104 chemical sciences 010404 medicinal & biomolecular chemistry Piperine biology.protein Cancer research Neoplastic Stem Cells Drug Screening Assays Antitumor |
| Zdroj: | Bioorganic chemistry. 110 |
| ISSN: | 1090-2120 |
| Popis: | Cancer stem cells (CSCs) are involved in recurrent hepatocellular carcinoma (HCC), yet there is a lack of effective treatment that targets these CSCs. CD44+ and CD133+ CSCs are markedly expressed in HepG2 cells and were isolated and characterized using fluorescence-activated cell sorting (FACS) analysis. Since piperine is known as an effective molecule against metastasis, we thought to investigate the effect of piperine against CD44+/CD133+ CSCs. Herein, piperine was found to be active against these CSCs. Also, it was found appropriate to respite at the 'subG0/G1 and G0/G1′ phase of the cell cycle analysis, respectively. TGF-β activated epithelial-mesenchymal transition (EMT) has been involved in the invasion and metastasis of HepG2 cells in hepatocellular carcinoma. Therefore, we next investigated the effect of piperine on different biomarkers that remarkably takes part in the process of EMT using flow cytometric analysis. Piperine was found able to repress the epithelial marker (E-cadherin) but was unable to restore the level of Vimentin (mesenchymal marker) and SNAIL (EMT-inducing transcription factor). Therefore, the findings of this study revealed that piperine could be an effective treatment strategy for recurrent hepatocarcinogenesis. |
| Databáze: | OpenAIRE |
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