Analysis of biological noise in the flagellar length control system
| Autor: | Kimberly A. Wemmer, Hiroaki Ishikawa, Jane Kondev, David P. Bauer, Nathan L. Hendel, Wallace F. Marshall |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Science 1.1 Normal biological development and functioning Biophysics 02 engineering and technology Flagellum Article 03 medical and health sciences Intraflagellar transport Mathematical Biosciences Underpinning research Organelle Size Molecular Biology Multidisciplinary biology Chemistry Chlamydomonas Cell Biology Biological Sciences 021001 nanoscience & nanotechnology Biological noise biology.organism_classification 030104 developmental biology Tubulin Control system biology.protein 0210 nano-technology Noise (radio) |
| Zdroj: | iScience, vol 24, iss 4 iScience, Vol 24, Iss 4, Pp 102354-(2021) iScience |
| Popis: | Summary Any proposed mechanism for organelle size control should be able to account not only for average size but also for the variation in size. We analyzed cell-to-cell variation and within-cell variation of length for the two flagella in Chlamydomonas, finding that cell-to-cell variation is dominated by cell size, whereas within-cell variation results from dynamic fluctuations. Fluctuation analysis suggests tubulin assembly is not directly coupled with intraflagellar transport (IFT) and that the observed length fluctuations reflect tubulin assembly and disassembly events involving large numbers of tubulin dimers. Length variation is increased in long-flagella mutants, an effect consistent with theoretical models for flagellar length regulation. Cells with unequal flagellar lengths show impaired swimming but improved gliding, raising the possibility that cells have evolved mechanisms to tune biological noise in flagellar length. Analysis of noise at the level of organelle size provides a way to probe the mechanisms determining cell geometry. Graphical abstract Highlights • Chlamydomonas flagella show dynamic length fluctuations • Length variation involves processes inside the cell body and in the flagella • Long-flagella mutants have increased length variability • Flagellar length variation leads to variation in cell motility Biological Sciences; Molecular Biology; Cell Biology; Biophysics; Mathematical Biosciences |
| Databáze: | OpenAIRE |
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