BAFF/BAFF-R involved in antibodies production of rats with collagen-induced arthritis via PI3K-Akt-mTOR signaling and the regulation of paeoniflorin

Autor: Shan Shan Song, Lingling Zhang, Dan Dan Liu, Wei Wei, Wen Di Zhao, Jing Yu Chen, Pei Pei Li, Yan Chang, Qingtong Wang, Yu Jing Wu, Yun Jie Liu
Rok vydání: 2011
Předmět:
Male
Time Factors
Anti-Inflammatory Agents
Arthritis
Pharmacology
Paeonia
Benzoates
Plant Roots
Rats
Sprague-Dawley
Phosphatidylinositol 3-Kinases
Glucosides
Drug Discovery
B-Cell Activating Factor
Phosphorylation
skin and connective tissue diseases
B-Lymphocytes
biology
TOR Serine-Threonine Kinases
medicine.anatomical_structure
Antibody
Signal Transduction
musculoskeletal diseases
Bridged-Ring Compounds
animal structures
Spleen
Immune system
stomatognathic system
medicine
Animals
B-cell activating factor
Protein kinase B
PI3K/AKT/mTOR pathway
Autoantibodies
Plants
Medicinal
business.industry
medicine.disease
Arthritis
Experimental
Rats
stomatognathic diseases
Immunology
biology.protein
Monoterpenes
business
Proto-Oncogene Proteins c-akt
Ankle Joint
B-Cell Activation Factor Receptor
Phytotherapy
Zdroj: Journal of ethnopharmacology. 141(1)
ISSN: 1872-7573
Popis: Ethnopharmacological relevance Paeoniflorin (Pae) is extracted from the root of paeonia lactiflora which have attracted attention for anti-rheumatic and immune modulating properties. Aim of the study To investigate the role of PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R in antibodies production and the regulation of Pae on the signaling pathway in rats with collagen-induced arthritis (CIA). Materials and methods CIA rats were randomly separated into different groups and treated with Pae (25, 100 mg/kg) from day 18 to day 38 after immunization. The effects of Pae on B lymphocytes of CIA rats were evaluated by the levels of BAFF, anti-CII antibody, IgA, IgG and IgM, and the expressions of BAFF-R, PI3K, p-Akt and mTOR. Results In CIA rats, the levels of anti-CII antibody, IgA, IgG and IgM in serum enhanced, BAFF, BAFF-R, PI3K, p-Akt and mTOR were highly expressed. Pae (100 mg/kg) obviously decreased arthritis score, relieved ankle and paw swelling, improved spleen histopathology in CIA rats, decreased the levels of IgA, IgM, IgG and anti-CII antibody, and significantly decreased the expressions of BAFF, BAFF-R, PI3K, p-Akt and mTOR. Conclusion PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R participates in antibodies production by B lymphocytes of CIA rats. Pae had therapeutic effects on rats with CIA. These effects might be relative to regulating PI3K/Akt/mTOR signal mediated by BAFF/BAFF-R, and down regulate the antibodies production further.
Databáze: OpenAIRE
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